Literature summary for 4.2.3.118 extracted from

Koeksal, M.; Chou, W.K.; Cane, D.E.; Christianson, D.W.
Unexpected reactivity of 2-fluorolinalyl diphosphate in the active site of crystalline 2-methylisoborneol synthase (2013), Biochemistry, 52, 5247-5255.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
in unliganded state and in complex with two Mg2+ ions and 2-fluoroneryl diphosphate at 1.85 and 2.00 A resolution, respectively. Cocrystallization with the fluorinated analogue of the tertiary allylic diphosphate intermediate, 2-fluorolinalyl diphosphate yields the crystal structure of the complex with the primary allylic diphosphate, 2-fluoroneryl diphosphate. The crystalline enzyme active site remains partially open, presumably due to the binding of only two Mg2+ ions	Streptomyces coelicolor

Crystallization (Comment)

Organism

in unliganded state and in complex with two Mg2+ ions and 2-fluoroneryl diphosphate at 1.85 and 2.00 A resolution, respectively. Cocrystallization with the fluorinated analogue of the tertiary allylic diphosphate intermediate, 2-fluorolinalyl diphosphate yields the crystal structure of the complex with the primary allylic diphosphate, 2-fluoroneryl diphosphate. The crystalline enzyme active site remains partially open, presumably due to the binding of only two Mg2+ ions

Streptomyces coelicolor

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	three Mg2+ ions are presumed to be required for the cyclization reaction	Streptomyces coelicolor

Organism

Organism	UniProt	Comment	Textmining
Streptomyces coelicolor	Q9F1Y6	-	-
Streptomyces coelicolor A3(2)	Q9F1Y6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2-fluorolinalyl diphosphate + H2O	-	Streptomyces coelicolor	(1R)-(+)-camphor + HF + diphosphate	single cyclic product, obtained via the (4R)-alpha-2-fluoroterpinyl carbocation intermediate. The initially generated 2-fluoroisoborneol cyclization product is unstable and undergoes elimination of hydrogen fluoride to yield (1R)-(+)-camphor	?
2-fluorolinalyl diphosphate + H2O	-	Streptomyces coelicolor A3(2)	(1R)-(+)-camphor + HF + diphosphate	single cyclic product, obtained via the (4R)-alpha-2-fluoroterpinyl carbocation intermediate. The initially generated 2-fluoroisoborneol cyclization product is unstable and undergoes elimination of hydrogen fluoride to yield (1R)-(+)-camphor	?
2-methyllinalyl diphosphate + H2O	natural intermediate of the cyclization reaction	Streptomyces coelicolor	2-methylisoborneol + diphosphate	-	?
2-methyllinalyl diphosphate + H2O	natural intermediate of the cyclization reaction	Streptomyces coelicolor A3(2)	2-methylisoborneol + diphosphate	-	?