Literature summary for 4.2.3.107 extracted from

Hall, D.E.; Robert, J.A.; Keeling, C.I.; Domanski, D.; Quesada, A.L.; Jancsik, S.; Kuzyk, M.A.; Hamberger, B.; Borchers, C.H.; Bohlmann, J.
An integrated genomic, proteomic and biochemical analysis of (+)-3-carene biosynthesis in Sitka spruce (Picea sitchensis) genotypes that are resistant or susceptible to white pine weevil (2011), Plant J., 65, 936-948.

Search for more literature for 4.2.3.107

Activating Compound

Activating Compound	Comment	Organism	Structure
methyl jasmonate	protein is detected at significantly higher levels in susceptible trees compared to resistant trees at day 7 after methyl jasmonate treatment	Picea sitchensis

Cloned(Commentary)

Cloned (Comment)	Organism
-	Picea sitchensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	isoform 3car3 has an extremely low activity	Picea sitchensis
0.00688	-	geranyl diphosphate	pH 7.4, 30°C, proteins from both susceptible and resistant plants have similar kinetic parameters	Picea sitchensis

Organism

Organism	UniProt	Comment	Textmining
Picea sitchensis	F1CKI6	isoform TPS-3car1, expressed both in trees susceptible and resistant to white pine weevil	-
Picea sitchensis	F1CKI8	isoform TPS-3car2, only expressed in trees resistant to white pine weevil	-
Picea sitchensis	F1CKI9	isoform TPS-3car3, only expressed in trees susceptilble to white pine weevil	-

Purification (Commentary)

Purification (Comment)	Organism
-	Picea sitchensis

Source Tissue

Source Tissue	Comment	Organism	Textmining
stem	-	Picea sitchensis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranyl diphosphate	-	Picea sitchensis	(+)-car-3-ene + diphosphate	major product, plus some terpinolene	?
additional information	poor substrate: neryl diphosphate. No substrates: farnesyl diphosphate, geranylgeranyl diphosphate	Picea sitchensis	?	-	?

Synonyms

Synonyms	Comment	Organism
TPS-3car1	-	Picea sitchensis
TPS-3car2	-	Picea sitchensis
TPS-3car3	-	Picea sitchensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.21	-	geranyl diphosphate	pH 7.4, 30°C, proteins from both susceptible and resistant plants have similar kinetic parameters	Picea sitchensis

Expression

Organism	Comment	Expression
Picea sitchensis	transcript levels are increased following methyl jasmonate treatment, with transcripts detected at significantly higher abundance in resistant trees compared to susceptible trees	up

General Information

General Information	Comment	Organism
physiological function	(+)-3-carene is associated with resistance of Sitka spruce to white pine weevil, Pissodes strobi. Variation of the (+)-3-carene phenotype is controlled by copy number variation of TPS-3car genes, variation of gene and protein expression, and variation in catalytic efficiencies	Picea sitchensis

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Release 2023.1 (January 2023)