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Literature summary for 4.2.3.106 extracted from

  • Arimura, G.; Ozawa, R.; Kugimiya, S.; Takabayashi, J;, Bohlmann, J.
    Herbivore-induced defense response in a model legume. Two-spotted spider mites induce emission of (E)-beta-ocimene and transcript accumulation of (E)-beta-ocimene synthase in Lotus japonicus (2004), Plant Physiol., 135, 1976-1983.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Lotus japonicus

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid N-terminal sequence indicates the presence of a plastid transit peptide Lotus japonicus 9536
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68800
-
 x * 68800, calculated Lotus japonicus

Organism

Organism UniProt Comment Textmining
Lotus japonicus Q672F7
-
-
Lotus japonicus Q672F7 deduced protein contains a modification of the conserved RRx8W motif of plant monoterpene synthases. The N-terminal sequence indicates the presence of a plastid transit peptide
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a plastid transit peptide Lotus japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate
-
 Lotus japonicus (E)-beta-ocimene + diphosphate products are 98% (E)-beta-ocimene plus 2% (Z)-beta-ocimene ?
geranyl diphosphate
-
 Lotus japonicus (E)-beta-ocimene + diphosphate 98% (E)-beta-ocimene plus 2% (Z)-beta-ocimene ?
additional information no substrates: farnesyl diphosphate or geranylgeranyl diphosphate. Lotus japonicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 68800, calculated Lotus japonicus

Synonyms

Synonyms Comment Organism
EbetaOS
-
 Lotus japonicus
EBOS
-
 Lotus japonicus

pI Value

Organism Comment pI Value Maximum pI Value
Lotus japonicus calculated
-
 6.3

Expression

Organism Comment Expression
Lotus japonicus induced in plants infested with two-spotted spider mites (Tetranychus urticae), coinciding with increasing emissions of (E)-beta-ocimene as well as other volatiles, (Z)-3-hexenyl acetate and (E)-4,8-dimethyl-1,3,7-nonatriene, by the infested plants. Mechanical wounding of the leaves or application of alamethicin also induces transiently increased levels of EbOS transcripts in Lotus japonicus. Wounding or alamethicin does not result in elevated release of (E)-beta-ocimene up
Lotus japonicus transcripts of LjEbOS are induced in plants infested with two-spotted spider mites, Tetranychus urticae, coinciding with increasing emissions of (E)-beta-ocimene as well as other volatiles, (Z)-3-hexenyl acetate and (E)-4,8-dimethyl-1,3,7-nonatriene, by the infested plants. Mechanical wounding of the leaves or application of alamethicin, a potent fungal elicitor of plant volatile emission, also induces transiently increased levels of transcripts, but not elevated release of (E)-beta-ocimene up

General Information

General Information Comment Organism
physiological function enzyme is involved in the herbivore-induced indirect defense response of spider mite-infested Lotus japonicus via de novo formation and emission (E)-beta-ocimene Lotus japonicus