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Literature summary for 4.2.3.101 extracted from

  • Koellner, T.; Schnee, C.; Gershenzon, J.; Degenhardt, J.
    The variability of sesquiterpenes emitted from two Zea mays cultivars is controlled by allelic variation of two terpene synthase genes encoding stereoselective multiple product enzymes (2004), Plant Cell, 16, 1115-1131.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.101

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE) cells Zea mays

Protein Variants

Protein Variants Comment Organism
E391Q the mutation is located in the vicinity of the catalytic center but does not influence enzymatic activity Zea mays
E455V the mutation results in an increase of catalytic activity Zea mays
I479T the mutation is located in the vicinity of the catalytic center but does not influence enzymatic activity Zea mays
K402Q the mutation is located in the vicinity of the catalytic center but does not influence enzymatic activity Zea mays
T383S the mutation is located in the vicinity of the catalytic center but does not influence enzymatic activity Zea mays
W163R the mutation restores much of the wild type activity (albeit maintaining the increased concentration of (E)-beta-farnesene relative to that of sesquithujene and (S)-beta-bisabolene) Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 geranyl diphosphate 20 mM MgCl2, 0.2 mM MnCl2, 0.2 mM NaWO4, and 0.1 mM NaF, pH not specified in the publication, at 30°C Zea mays
0.0011
-
 (2E,6E)-farnesyl diphosphate 20 mM MgCl2, 0.2 mM MnCl2, 0.2 mM NaWO4, and 0.1 mM NaF, pH not specified in the publication, at 30°C Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ either Mg2+ or Mn2+ is required for activity, the optimum is 5 mM Mg2+ Zea mays
Mn2+ either Mg2+ or Mn2+ is required for activity, the optimum is 0.25 mM Mg2+ Zea mays
additional information no enzyme activity in the presence of Ca2+, Cd2+, Co2+, Cu2+, Ni2+, and Zn2+ Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63960
-
 calculated from amino acid sequence Zea mays
66000
-
 gel filtration Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
 inbred line B73
-

Purification (Commentary)

Purification (Comment) Organism
Econopac 10DG column chromatography and Strep-Tactine affinity column chromatography Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
husk highest expression Zea mays
-
leaf ear leaf Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Zea mays 7-epi-sesquithujene + diphosphate 7-epi-sesquithujene is the main product, the enzyme also produces (S)-beta-bisabolene, (E)-beta-farnesene, and the minor products sesquithujene, (Z)-alpha-bergamotene, sesquisabinene B, (E)-alpha-bergamotene, and sesquisabinene A ?
geranyl diphosphate
-
 Zea mays (S)-(-)-limonene + diphosphate main product is (S)-(-)-limonene, minor products are alpha-thujene, beta-myrcene, (+)-sabinene, (-)-sabinene, alpha-terpinene, (E)-beta-ocimene, beta-phellandrene, alpha-terpinolene, gamma-terpinene, sabinene hydrate, (R)-(-)-linalool, and (S)-(1)-linalool ?

Synonyms

Synonyms Comment Organism
TPS4
-
 Zea mays
TPS4-B73
-
 Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Zea mays

pH Range

pH Minimum pH Maximum Comment Organism
6.3 8.2
-
 Zea mays