Activating Compound | Comment | Organism | Structure |
---|---|---|---|
S-adenosylmethionine | affects both Km and Vmax by allosteric and cooperative transition of enzyme, two mol per mol enzyme, 25fold decrease of Km at 0.06 mM | Arabidopsis thaliana |
Application | Comment | Organism |
---|---|---|
agriculture | possible herbicide target | Arabidopsis thaliana |
nutrition | interesting with respect to attempts to obtain transgenic plants with elevated levels of essential amino acids Met, Lys, Thr | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
gel filtration | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Arabidopsis thaliana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
O-phospho-L-homoserine + H2O = L-threonine + phosphate | mechanism | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.8 | - |
- |
Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
-80°C, Na-HEPES buffer, pH 7.5, several months, no loss of activity | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-phospho-L-homoserine + H2O | - |
Arabidopsis thaliana | L-threonine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
enzyme assay at | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
O-phosphohomoserine | without S-adenosylmethionine | Arabidopsis thaliana | |
3.5 | - |
O-phosphohomoserine | at 0.06 mM S-adenosylmethionine | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
enzyme assay at | Arabidopsis thaliana |