Literature summary for 4.2.3.1 extracted from

Greenberg, J.M.; Thompson, J.F.; Madison, J.T.
Homoserine kinase and threonine synthase in methionine-overproducing soybean tissue cultures (1988), Plant Cell Rep., 7, 477-480.

Search for more literature for 4.2.3.1

Inhibitors

Inhibitors	Comment	Organism	Structure
L-cysteine	strong inhibition at 0.5 mM	Glycine max

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	not exclusively in stroma	Glycine max	9507	-

Organism

Organism	UniProt	Comment	Textmining
Glycine max	-	wild type and several methionine-overproducing strains	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Glycine max	-
cell suspension culture	-	Glycine max	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	activity with addition of several amino acids to several mutant cell lines	Glycine max

Storage Stability

Storage Stability	Organism
-80°C, MOPS-buffer, pH 7.5, EDTA, dithioerythritol, 2-benzothiazolethiol, glycerol, polyvinylpyrrolidone, several months, no loss of activity	Glycine max

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
O-phospho-L-homoserine + H2O	-	Glycine max	L-threonine + phosphate	-	ir

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	enzyme assay at	Glycine max

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.3	-	enzyme assay at	Glycine max

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Release 2023.1 (January 2023)