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Literature summary for 4.2.3.1 extracted from

  • Curien, G.; Dumas, R.; Ravanel, S.; Douce, R.
    Characterization of an Arabidopsis thaliana cDNA encoding an S-adenosylmethionine-sensitive threonine synthase. Threonine synthase from higher plants (1996), FEBS Lett., 390, 85-90.
    View publication on PubMed
Search for more literature for 4.2.3.1

Activating Compound

Activating Compound Comment Organism Structure
S-adenosylmethionine 3-20fold increase of specific activity in various recombinant enzymes at 0.2 mM Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 2 * 58000, comparison of nucleotide sequence and molecular mass of native enzyme Arabidopsis thaliana
110000
-
 gel filtration Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant enzymes Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00098 0.00246 purified enzyme from cloned gene Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-phospho-L-homoserine + H2O
-
 Arabidopsis thaliana L-threonine + phosphate
-
 ir

Subunits

Subunits Comment Organism
dimer 2 * 58000, comparison of nucleotide sequence and molecular mass of native enzyme Arabidopsis thaliana
More identification of active site amino acids Arabidopsis thaliana