Literature summary for 4.2.3.1 extracted from

Giovanelli, J.; Veluthambi, K.; Thompson, G.A.; Mudd, S.H.; Datko, A.H.
Threonine synthase of Lemna paucicostata Hegelm. 6746 (1984), Plant Physiol., 76, 285-292.

Search for more literature for 4.2.3.1

Activating Compound

Activating Compound	Comment	Organism	Structure
S-adenosylmethionine	allosteric activator, maximal activation at 0.1-0.2 mM	Lemna aequinoctialis

General Stability

General Stability	Organism
bovine serum albumine stabilizes	Lemna aequinoctialis

Inhibitors

Inhibitors	Comment	Organism	Structure
L-cysteine	26% inhibition at 1 mM, reversible	Lemna aequinoctialis
O-phospho-DL-threonine	-	Lemna aequinoctialis
O-phospho-L-serine	-	Lemna aequinoctialis
Vinylglycine	22% inhibition at 10 mM	Lemna aequinoctialis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.002	0.007	O-phospho-L-homoserine	at saturating S-adenosylmethionine concentrations	Lemna aequinoctialis

Organism

Organism	UniProt	Comment	Textmining
Lemna aequinoctialis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Lemna aequinoctialis

Reaction

Reaction	Comment	Organism	Reaction ID
O-phospho-L-homoserine + H2O = L-threonine + phosphate	mechanism	Lemna aequinoctialis	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0261	-	-	Lemna aequinoctialis

Storage Stability

Storage Stability	Organism
-80°C, pH 7.8, several months	Lemna aequinoctialis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
O-phospho-L-homoserine + H2O	-	Lemna aequinoctialis	L-threonine + phosphate	-	ir

Subunits

Subunits	Comment	Organism
More	amino acid analysis	Lemna aequinoctialis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Lemna aequinoctialis

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	8.5	-	Lemna aequinoctialis

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Release 2023.1 (January 2023)