Cloned (Comment) | Organism |
---|---|
mutant enzyme E64Q is expressed in Escherichi coli strain B834(DE3) pLysS | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
in complex with the glycopeptide inhibitor bulgecin A and N-acetylglucosaminyl-N-acetylmuramyl-L-Ala-D-Glu, hanging drop vapor diffusion method, using 0.1 M lithium sulfate, 15% (w/v) PEG 8000 in 20 mM HEPES buffer, pH 7.5 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E64Q | inactive | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | a periplasmic, outer membrane-attached enzyme | Escherichia coli | 16020 | - |
periplasm | - |
Escherichia coli | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0C960 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Micrococcus luteus peptidoglycan | - |
Escherichia coli | ? | - |
? | |
additional information | the enzyme cleaves the beta-1,4-glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine residues in the cell wall peptidoglycan, producing 1,6-anhydromuropeptides | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | in solution, the enzyme behaves as a monomer, as judged from size exclusion chromatography and dynamic light scattering | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
endolytic peptidoglycan lytic transglycosylase | - |
Escherichia coli |
GlcNAc-beta-(1->4)-Mur2Acoyl-L-Ala-gamma-D-Glu lyase (1,6-anhydromuramoyl-residue forming) | systematic name | Escherichia coli |
MltE | - |
Escherichia coli |
muropeptide lyase | recommended name | Escherichia coli |