Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pedobacter heparinus |
Protein Variants | Comment | Organism |
---|---|---|
I133T/P316T | mutant displays about 7fold increase in catalytic efficiency | Pedobacter heparinus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, about 30% inhibition | Pedobacter heparinus | |
Mg2+ | 1 mM, about 30% inhibition | Pedobacter heparinus | |
Mn2+ | 1 mM, about 30% inhibition | Pedobacter heparinus | |
Ni2+ | 1 mM, about 30% inhibition | Pedobacter heparinus | |
Zn2+ | 1 mM, about 30% inhibition | Pedobacter heparinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
heparin | mutant I133T/P316T, pH 7.5, 40°C | Pedobacter heparinus | |
0.56 | - |
heparin | wild-type, pH 7.5, 40°C | Pedobacter heparinus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 57% increase in activity for mutant I133T/P316T | Pedobacter heparinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pedobacter heparinus | B3U3D9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
heparin | - |
Pedobacter heparinus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
heparinase I | - |
Pedobacter heparinus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21 | - |
heparin | wild-type, pH 7.5, 40°C | Pedobacter heparinus | |
27 | - |
heparin | mutant I133T/P316T, pH 7.5, 40°C | Pedobacter heparinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pedobacter heparinus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | almost no activity for wild-type | Pedobacter heparinus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
heparin | wild-type, pH 7.5, 40°C | Pedobacter heparinus | |
0.27 | - |
heparin | mutant I133T/P316T, pH 7.5, 40°C | Pedobacter heparinus |