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Literature summary for 4.2.2.7 extracted from

  • Ma, X.; Wang, Z.; Li, S.; Shen, Q.; Yuan, Q.
    Effect of CaCl2 as activity stabilizer on purification of heparinase I from Flavobacterium heparinum (2006), J. Chromatogr. B, 843, 209-215.
    View publication on PubMed
Search for more literature for 4.2.2.7

General Stability

General Stability Organism
CaCl2 is a good stabilizer of the purified enzyme in liquid form toward either storaging at 4°C or freezing-thawing Pedobacter heparinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Pedobacter heparinus

Organism

Organism UniProt Comment Textmining
Pedobacter heparinus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pedobacter heparinus

Storage Stability

Storage Stability Organism
4°C, in the presence of CaCl2 15% loss of activity after 5 days Pedobacter heparinus

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Pedobacter heparinus

Synonyms

Synonyms Comment Organism
heparinase I
-
 Pedobacter heparinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
41
-
-
 Pedobacter heparinus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15
-
 30 min, stable Pedobacter heparinus
35
-
 30 min, rapid inactivation at 35°C or above Pedobacter heparinus
55
-
 30 min, nearly complete inactivation Pedobacter heparinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pedobacter heparinus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 11 0°C, 30 min, stable. Inactivated out of this range Pedobacter heparinus

pI Value

Organism Comment pI Value Maximum pI Value
Pedobacter heparinus isoelectric focusing
-
 8.5