Any feedback?
Literature summary for 4.2.2.7 extracted from
- High yield, purity and activity of soluble recombinant Bacteroides thetaiotaomicron GST-heparinase I from Escherichia coli (2007), Arch. Biochem. Biophys., 460, 17-24.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | catalytic activity of heparinase I requires a reducing environment | Bacteroides thetaiotaomicron |
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the GSTfused enzyme with high purity and specific activity may be useful in solid phase clinical applications and sequence characterizations of structurally specific heparin and heparan sulfate motifs | Bacteroides thetaiotaomicron |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli as a fusion protein with GST at the N-terminus | Bacteroides thetaiotaomicron |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0023 | - |
heparin | - |
Bacteroides thetaiotaomicron |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | purified GST fusion enzyme requires Ca2+. Activity of the GST-heparinase I is stimulated 7fold by 1 mM Ca2+. Mg2+ and Mn2+ are much less effective at the same concentrations while Cu2+ and Zn2+ are ineffective | Bacteroides thetaiotaomicron | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteroides thetaiotaomicron | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme expressed in Escherichia coli as a fusion protein with GST at the N-terminus, one-step affinity purification | Bacteroides thetaiotaomicron |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| heparin | - |
Bacteroides thetaiotaomicron | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| heparinase I | - |
Bacteroides thetaiotaomicron |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 7.3 | - |
Bacteroides thetaiotaomicron |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 7.9 | pH 5.8: about 55% of maximal activity, pH 7.9: about 55% of maximal activity | Bacteroides thetaiotaomicron |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
