Cloned (Comment) | Organism |
---|---|
expression of the recombinant wild type and several mutants in Escherichia coli | Pedobacter heparinus |
Protein Variants | Comment | Organism |
---|---|---|
D210A | mutation does not affect the product profile compared to the wild type anzyme | Pedobacter heparinus |
D212A | mutation does not affect the product profile compared to the wild type anzyme | Pedobacter heparinus |
E207A | mutation does not affect the product profile compared to the wild type anzyme | Pedobacter heparinus |
E381A | produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments | Pedobacter heparinus |
G213A | mutation does not affect the product profile compared to the wild type anzyme | Pedobacter heparinus |
G378A | produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments | Pedobacter heparinus |
additional information | construction of several double and triple mutants of the calcium-coordinating residues | Pedobacter heparinus |
N375A | produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments | Pedobacter heparinus |
S377A | produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments | Pedobacter heparinus |
T216A | mutation does not affect the product profile compared to the wild type anzyme | Pedobacter heparinus |
T373A | produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments | Pedobacter heparinus |
T382A | produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments | Pedobacter heparinus |
Y379A | produces lower amounts of disaccharides and tetrasaccharides and a greater fraction of larger digestion fragments | Pedobacter heparinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
heparin | several double and triple mutants of the calcium-coordinating residues | Pedobacter heparinus | |
0.003 | - |
heparin | Y379A mutant, pH 7.0 30ºC | Pedobacter heparinus | |
0.005 | - |
heparin | E381A mutant, pH 7.0 30ºC | Pedobacter heparinus | |
0.007 | - |
heparin | G378A mutant, pH 7.0 30ºC | Pedobacter heparinus | |
0.009 | - |
heparin | N375A mutant, pH 7.0 30ºC | Pedobacter heparinus | |
0.01 | - |
heparin | G213A mutant, pH 7.0 30ºC | Pedobacter heparinus | |
0.01 | - |
heparin | T373A mutant, pH 7.0 30ºC | Pedobacter heparinus | |
0.015 | - |
heparin | T216A mutant, pH 7.0 30ºC | Pedobacter heparinus | |
0.016 | - |
heparin | T382A mutant, pH 7.0 30ºC | Pedobacter heparinus | |
0.019 | - |
heparin | D212A mutant, pH 7.0 30ºC | Pedobacter heparinus | |
0.026 | - |
heparin | D210A mutant, pH 7.0 30ºC | Pedobacter heparinus | |
0.027 | - |
heparin | S377A mutant, pH 7.0 30ºC | Pedobacter heparinus | |
0.031 | - |
heparin | E207A mutant, pH 7.0 30ºC | Pedobacter heparinus | |
0.039 | - |
heparin | wild type recombinant enzyme, pH 7.0 30ºC | Pedobacter heparinus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | essential for activity, two calcium-binding sites with different affinity | Pedobacter heparinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pedobacter heparinus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
heparin | - |
Pedobacter heparinus | unsaturated heparin disaccharide + unsaturated heparin tetrasaccharide + unsaturated heparin hexasaccharide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
heparinase I | - |
Pedobacter heparinus |