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Literature summary for 4.2.2.7 extracted from

  • Kim, B.T.; Kim, W.S.; Kim, Y.S.; Linhardt, R.J.; Kim, D.H.
    Purification and characterization of a novel heparinase from Bacteroides stercoris HJ-15 (2000), J. Biochem., 128, 323-328.
    View publication on PubMed
Search for more literature for 4.2.2.7

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 90% inhibition at 0.1 mM Bacteroides stercoris
p-chloromercuriphenyl sulfonic acid complete inhibition at 0.05 mM Bacteroides stercoris
Pb2+ 80% inhibition at 0.1 mM Bacteroides stercoris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 heparan sulfate pH 7.2, 40ºC Bacteroides stercoris
0.09
-
 heparin pH 7.2, 40ºC Bacteroides stercoris

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ slight activation Bacteroides stercoris
Ca2+ slight activation Bacteroides stercoris
K+ up to 300 mM Bacteroides stercoris
Mg2+ slight activation Bacteroides stercoris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 gel filtration Bacteroides stercoris

Organism

Organism UniProt Comment Textmining
Bacteroides stercoris
-
 HJ-15 strain
-
Bacteroides stercoris HJ-15
-
 HJ-15 strain
-

Purification (Commentary)

Purification (Comment) Organism
by several chromatographic steps, including QAE-cellulose, DEAE-cellulose, Sephadex and hydroxyapatite Bacteroides stercoris

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.5
-
 pH 7.2, 40ºC Bacteroides stercoris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heparan sulfate 600% activity compared to heparin Bacteroides stercoris ?
-
 ?
heparan sulfate 600% activity compared to heparin Bacteroides stercoris HJ-15 ?
-
 ?
heparin
-
 Bacteroides stercoris ?
-
 ?
heparin
-
 Bacteroides stercoris HJ-15 ?
-
 ?
additional information chondroitin sulfate A,B or C, and hyaluronic acid can not act as substrates Bacteroides stercoris ?
-
 ?
additional information chondroitin sulfate A,B or C, and hyaluronic acid can not act as substrates Bacteroides stercoris HJ-15 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Bacteroides stercoris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 pH 7.2 Bacteroides stercoris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 45ºC Bacteroides stercoris

pH Range

pH Minimum pH Maximum Comment Organism
6 8.4 10% of the maximal activity below and above this range Bacteroides stercoris