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Literature summary for 4.2.2.6 extracted from
- Oligogalacturonide trans-eliminase of Erwinia carotovora (1968), Arch. Biochem. Biophys., 125, 734-741.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
unsaturated digalacturonic acid | - |
Erwinia aroidea |  |
| 5.3 | - |
digalacturonic acid | - |
Erwinia aroidea | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Erwinia aroidea | two pathways are involved in metabolism of pectic substances: one route through galacturonic acid, involving galacturonic acid isomerase and the other through unsaturated digalacturonic acid, involving EC 4.2.2.5, with the formation of 4-deoxy-5-hexosulose uronic acid. Both pathways yield 2-keto-3-deoxy-D-gluconic acid as common product | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Erwinia aroidea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Erwinia aroidea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Erwinia aroidea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-(4-deoxy-alpha-L-threo-hex-4-enopyranuronosyl)-beta-D-galactopyranuronic acid | i.e. O-(4-deoxy-beta-L-5-threo-hexopyranos-4-enyluronic acid)-(1,4)-D-galacturonic acid | Erwinia aroidea | 4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid + D-galacturonic acid | - |
? | |
| digalacturonic acid | - |
Erwinia aroidea | 4-deoxy-5-keto-D-fructuronic acid + ? | - |
? | |
| additional information | two pathways are involved in metabolism of pectic substances: one route through galacturonic acid, involving galacturonic acid isomerase and the other through unsaturated digalacturonic acid, involving EC 4.2.2.5, with the formation of 4-deoxy-5-hexosulose uronic acid. Both pathways yield 2-keto-3-deoxy-D-gluconic acid as common product | Erwinia aroidea | ? | - |
? | |
| unsaturated digalacturonic acid | - |
Erwinia aroidea | 4-deoxy-5-keto-D-fructuronic acid + ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Erwinia aroidea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | pH 6.5: about 45% of maximal activity, pH 8.0: about 55% of maximal activity | Erwinia aroidea |
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Release 2023.1 (January 2023)
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