Cloned (Comment) | Organism |
---|---|
gene algL, expression from pNMAlgL28362 expression vector as His-tagged enzyme in Escherichia coli strain Origami 2 (DE3) | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged AlgL28-362 protein, hanging drop vapour diffusion method, mixing of 0.002 ml of 8 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, with 0.002 ml of reservoir solution containing 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate, pH 4.6, 28% w/v PEG 4000, 0.3 M PIPES, method optimization, 4 days, 20°C, X-ray diffraction structure determination and analysis at 1.64 A resolution | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000 | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | AlgL is a poly-(beta-D-mannuronate) lyase that preferentially degrades deacetylated polymannuronate via a beta-elimination reaction, resulting in an unsaturated uronic acid at the nonreducing end of the molecule | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q06749 | gene algL, encoded on the algD operon, AlgD/8/44/K/E/G/X/L/I/J/F/A | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged AlgL28-362 protein from Escherichia coli strain Origami 2 (DE3) by nickel affinity chrmatography, ultrafiltration, and gel filtration | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | AlgL is a poly-(beta-D-mannuronate) lyase that preferentially degrades deacetylated polymannuronate via a beta-elimination reaction, resulting in an unsaturated uronic acid at the nonreducing end of the molecule | Pseudomonas aeruginosa | ? | - |
? | |
additional information | AlgL is an endolytic enzyme that cleaves the 1-4 glycosidic linkage, resulting in disaccharides and trisaccharides as its major products. Several residues, including His202 and Tyr256, play a role in AlgL activity | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000 | Pseudomonas aeruginosa |
More | AlgL has an alpha6/alpha5-barrel fold | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
AlgL | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
evolution | AlgL belong to the polysaccharide family 5 | Pseudomonas aeruginosa |
metabolism | the enzyme is involved in the biosynthesis of alginate | Pseudomonas aeruginosa |