Application | Comment | Organism |
---|---|---|
molecular biology | this is the first report that yeast cells displaying alginate lyase are used to produce different lengths of oligosaccharides from alginate | Vibrio sp. |
Cloned (Comment) | Organism |
---|---|
gene of alginate lyase (AlyVI) from marine bacterium Vibrio is cloned into the multiple cloning site of the surface display vector pINA1317-YlCWP110 and expressed in cells of Yarrowia lipolytica | Vibrio sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | - |
Vibrio sp. | ? | - |
? | |
poly-alpha-L-guluronic acid | - |
Vibrio sp. | ? | - |
? | |
poly-beta-D-mannuronate | poly-beta-D-mannuronate can be much more easily hydrolyzed by the displayed alginate lyase than poly-alpha-L-guluronic acid and sodium alginate | Vibrio sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alginate lyase | - |
Vibrio sp. |
AlyVI | - |
Vibrio sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Vibrio sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Vibrio sp. |
7.3 | - |
maximal activity | Vibrio sp. |