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Literature summary for 4.2.2.3 extracted from
- Cloning and Characterization of Alginate Lyase from a Marine Bacterium Streptomyces sp. ALG-5 (2009), Mar. Biotechnol., 11, 10-16.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as a His-tagged fusion protein in Escherichia coli | Streptomyces sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27500 | - |
SDS-PAGE | Streptomyces sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces sp. | A7LFP7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-Sepharose affinity chromatography | Streptomyces sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate | enzyme lyase appears to be poly-guluronate lyase degrading poly-G block preferentially than poly-M block. The degraded products are determined to be di-, tri-, tetra- and pentasaccharides | Streptomyces sp. | unsaturated algino-oligosaccharides | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ALG-5 | - |
Streptomyces sp. |
| alginate lyase | - |
Streptomyces sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Streptomyces sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Streptomyces sp. |
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Release 2023.1 (January 2023)
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