Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Haliotis discus hannai |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | Haliotis discus hannai |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haliotis discus hannai | - |
- |
- |
Haliotis discus hannai | Q25C77 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Haliotis discus hannai |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
digestive juice | - |
Haliotis discus hannai | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1786 | - |
pH 7.0, 30°C | Haliotis discus hannai |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | HdAlex can degrade not only unsaturated trisaccharide but also alginate and mannuronate-rich polymers. HdAlex degrades the alginate polymer in an exolytic manner | Haliotis discus hannai | ? | - |
? | |
alginate + H2O | - |
Haliotis discus hannai | ? | - |
? | |
poly(beta-(1->4)-D-mannuronate) | 530% of the activity with alginate | Haliotis discus hannai | an unsaturated mannuronate disaccharide | enzyme acts as an exolytic lyase, releasing unsaturated disaccharide directly from the poly(M)-rich substrate. It splits the second glycosyl linkage from the reducing terminus of the trisaccharide, the resulting disaccharide is not further degraded | ? | |
poly(beta-(1->4)-D-mannuronic acid/alpha-(1->4)-L-guluronic acid) | 45% of the activity with alginate | Haliotis discus hannai | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Haliotis discus hannai |
Synonyms | Comment | Organism |
---|---|---|
HdAlex | - |
Haliotis discus hannai |
hdalex-1 | - |
Haliotis discus hannai |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
- |
Haliotis discus hannai |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 30°C: about 50% of maximal activity, 50°C: about 10% of maximal activity | Haliotis discus hannai |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
- |
Haliotis discus hannai |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | pH 5.5: about 30% of maximal activity, pH 8.0: about 60% of maximal activity | Haliotis discus hannai |
General Information | Comment | Organism |
---|---|---|
physiological function | the preferable substrate of enzyme Hdalex in the abalone digestive fluid is the unsaturated trisaccharide produced by alginate lyase Hdaly, EC 4.2.2.3 | Haliotis discus hannai |