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Literature summary for 4.2.2.3 extracted from

  • Suzuki, H.; Suzuki, K.; Inoue, A.; Ojima, T.
    A novel oligoalginate lyase from abalone, Haliotis discus hannai, that releases disaccharide from alginate polymer in an exolytic manner (2006), Carbohydr. Res., 341, 1809-1819.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Haliotis discus hannai

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
x * 32000, SDS-PAGE Haliotis discus hannai

Organism

Organism UniProt Comment Textmining
Haliotis discus hannai
-
-
-
Haliotis discus hannai Q25C77
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Haliotis discus hannai

Source Tissue

Source Tissue Comment Organism Textmining
digestive juice
-
Haliotis discus hannai
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1786
-
pH 7.0, 30°C Haliotis discus hannai

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate HdAlex can degrade not only unsaturated trisaccharide but also alginate and mannuronate-rich polymers. HdAlex degrades the alginate polymer in an exolytic manner Haliotis discus hannai ?
-
?
alginate + H2O
-
Haliotis discus hannai ?
-
?
poly(beta-(1->4)-D-mannuronate) 530% of the activity with alginate Haliotis discus hannai an unsaturated mannuronate disaccharide enzyme acts as an exolytic lyase, releasing unsaturated disaccharide directly from the poly(M)-rich substrate. It splits the second glycosyl linkage from the reducing terminus of the trisaccharide, the resulting disaccharide is not further degraded ?
poly(beta-(1->4)-D-mannuronic acid/alpha-(1->4)-L-guluronic acid) 45% of the activity with alginate Haliotis discus hannai ?
-
?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Haliotis discus hannai

Synonyms

Synonyms Comment Organism
HdAlex
-
Haliotis discus hannai
hdalex-1
-
Haliotis discus hannai

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
Haliotis discus hannai

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 30°C: about 50% of maximal activity, 50°C: about 10% of maximal activity Haliotis discus hannai

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
-
Haliotis discus hannai

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 pH 5.5: about 30% of maximal activity, pH 8.0: about 60% of maximal activity Haliotis discus hannai

General Information

General Information Comment Organism
physiological function the preferable substrate of enzyme Hdalex in the abalone digestive fluid is the unsaturated trisaccharide produced by alginate lyase Hdaly, EC 4.2.2.3 Haliotis discus hannai