Cloned (Comment) | Organism |
---|---|
gene cl2, DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His-tagged protein | Chlorella virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Chlorella virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for activity | Chlorella virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, recombinant enzyme, SDS-PAGE | Chlorella virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorella virus | Q9QT64 | isolated from Chlorella sp. strain NC64A, gene cl2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli, 4.8fold | Chlorella virus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.021 | - |
purified recombinant enzyme | Chlorella virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | - |
Chlorella virus | ? | - |
? | |
additional information | no activity with chitin, chitosan, cellulose, and pectin | Chlorella virus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, recombinant enzyme, SDS-PAGE | Chlorella virus |
Synonyms | Comment | Organism |
---|---|---|
alginate lyase | - |
Chlorella virus |
CL2 | - |
Chlorella virus |
Lysis protein | - |
Chlorella virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Chlorella virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
- |
Chlorella virus |