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Literature summary for 4.2.2.3 extracted from

  • Suda, K.; Tanji, Y.; Hori, K.; Unno, H.
    Evidence for a novel Chlorella virus-encoded alginate lyase (1999), FEMS Microbiol. Lett., 180, 45-53.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene cl2, DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His-tagged protein Chlorella virus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Chlorella virus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for activity Chlorella virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
x * 39000, recombinant enzyme, SDS-PAGE Chlorella virus

Organism

Organism UniProt Comment Textmining
Chlorella virus Q9QT64 isolated from Chlorella sp. strain NC64A, gene cl2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli, 4.8fold Chlorella virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.021
-
purified recombinant enzyme Chlorella virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
Chlorella virus ?
-
?
additional information no activity with chitin, chitosan, cellulose, and pectin Chlorella virus ?
-
?

Subunits

Subunits Comment Organism
? x * 39000, recombinant enzyme, SDS-PAGE Chlorella virus

Synonyms

Synonyms Comment Organism
alginate lyase
-
Chlorella virus
CL2
-
Chlorella virus
Lysis protein
-
Chlorella virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Chlorella virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
Chlorella virus