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Literature summary for 4.2.2.3 extracted from

  • Ertesvag, H.; Erlien, F.; Skjak-Braek, G.; Rehm, B.H.A.; Valla, S.
    Biochemical properties and substrate specificities of a recombinantly produced Azotobacter vinelandii alginate lyase (1998), J. Bacteriol., 180, 3779-3784.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Azotobacter vinelandii

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ 1 mM ZnCl2 reduces the activity to 7.6% of the control level without Zn2+ added, 0.1 mM Zn2+ reduces the activity to 61% Azotobacter vinelandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
 alginate
-
 Azotobacter vinelandii
0.46
-
 alginate alginate rich in mannuronic acid Azotobacter vinelandii

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl activity is optimal at 0.35 M Azotobacter vinelandii
Zn2+ 1 mM ZnCl2, 92% loss of activity Azotobacter vinelandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 x * 39000, SDS-PAGE Azotobacter vinelandii

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-
Azotobacter vinelandii O52195 alginate lyase precursor; recombinant
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification signal peptide is cleaved off, leaving a mature protein of 39000 Da Azotobacter vinelandii

Purification (Commentary)

Purification (Comment) Organism
partial Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate alginate rich in mannuronic acids, the enzyme cleaves beta-D-mannuronic acid-beta-D-mannuronic acid bonds and beta-D-mannuronic acid-alpha-L-guluronic acid bonds but not alpha-L-guluronic acid-beta-D-mannuronic acid bonds and alpha-L-guluronic acid-alpha-L-guluronic acid-bonds Azotobacter vinelandii oligouronides
-
 ?
alginate enzyme cleaves beta-D-mannuronic acid-beta-D-mannuronic acid and beta-D-mannuronic acid-alpha-L-guluronic acid bonds but not alpha-L-guluronic acid-alpha-L-guluronic acid or alpha-L-guluronic acid-beta-D-mannuronic acid bonds Azotobacter vinelandii unsaturated algino-oligosaccharides
-
 ?

Subunits

Subunits Comment Organism
? x * 39000, SDS-PAGE Azotobacter vinelandii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1 8.4
-
 Azotobacter vinelandii

pH Range

pH Minimum pH Maximum Comment Organism
6.7 8.4 pH 6.7: about 50% of maximal activity, pH 8.1-8.4: optimum Azotobacter vinelandii

pI Value

Organism Comment pI Value Maximum pI Value
Azotobacter vinelandii isoelectric focusing
-
 5.1