Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Shewanella sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 1 mM, 56% residual activity | Shewanella sp. | |
Ca2+ | 1 mM, 80% residual activity | Shewanella sp. | |
EDTA | 1 mM, 49% residual activity | Shewanella sp. | |
Fe3+ | 1 mM, 44% residual activity | Shewanella sp. | |
Ni2+ | 1 mM, 40% residual activity | Shewanella sp. | |
SDS | 1 mM, 34% residual activity | Shewanella sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | 10 mM, 147% of initial activity, 300 mM, 276% of initial activity | Shewanella sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
x * 82000, SDS-PAGE | Shewanella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella sp. | A0A088CA41 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence contains a putative signal peptide of 44 amino acid residues | Shewanella sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5 | - |
substrate polyG, pH 7.0, 50°C | Shewanella sp. |
24 | - |
substrate polyM, pH 7.0, 50°C | Shewanella sp. |
32 | - |
substrate alginate, pH 7.0, 50°C | Shewanella sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate + H2O | - |
Shewanella sp. | ? | enzyme degrades alginate more efficiently than polyM and polyG block into a monomeric sugar acid | ? | |
poly(beta-(1->4)guluronate) + H2O | i.e. linear block polymer of alpha-L-guluronic acid | Shewanella sp. | ? | - |
? | |
poly(beta-(1->4)mannuronate) + H2O | i.e. linear block polymer of beta-D-mannuronic acid | Shewanella sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 82000, SDS-PAGE | Shewanella sp. |
Synonyms | Comment | Organism |
---|---|---|
oalS17 | - |
Shewanella sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Shewanella sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Shewanella sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Shewanella sp. |