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Literature summary for 4.2.2.26 extracted from

  • Wang,L.; Li, S.; Yu, W.; Gong, Q.
    Cloning, overexpression and characterization of a new oligoalginate lyase from a marine bacterium, Shewanella sp. (2015), Biotechnol. Lett., 37, 665-671.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Shewanella sp.

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1 mM, 56% residual activity Shewanella sp.
Ca2+ 1 mM, 80% residual activity Shewanella sp.
EDTA 1 mM, 49% residual activity Shewanella sp.
Fe3+ 1 mM, 44% residual activity Shewanella sp.
Ni2+ 1 mM, 40% residual activity Shewanella sp.
SDS 1 mM, 34% residual activity Shewanella sp.

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl 10 mM, 147% of initial activity, 300 mM, 276% of initial activity Shewanella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
x * 82000, SDS-PAGE Shewanella sp.

Organism

Organism UniProt Comment Textmining
Shewanella sp. A0A088CA41
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a putative signal peptide of 44 amino acid residues Shewanella sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5
-
substrate polyG, pH 7.0, 50°C Shewanella sp.
24
-
substrate polyM, pH 7.0, 50°C Shewanella sp.
32
-
substrate alginate, pH 7.0, 50°C Shewanella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate + H2O
-
Shewanella sp. ? enzyme degrades alginate more efficiently than polyM and polyG block into a monomeric sugar acid ?
poly(beta-(1->4)guluronate) + H2O i.e. linear block polymer of alpha-L-guluronic acid Shewanella sp. ?
-
?
poly(beta-(1->4)mannuronate) + H2O i.e. linear block polymer of beta-D-mannuronic acid Shewanella sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 82000, SDS-PAGE Shewanella sp.

Synonyms

Synonyms Comment Organism
oalS17
-
Shewanella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Shewanella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
Shewanella sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9
-
Shewanella sp.