Literature summary for 4.2.2.24 extracted from

Ochiai, A.; Itoh, T.; Mikami, B.; Hashimoto, W.; Murata, K.
Structural determinants responsible for substrate recognition and mode of action in family 11 polysaccharide lyases (2009), J. Biol. Chem., 284, 10181-10189.

Search for more literature for 4.2.2.24

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, structure determined at 1.32 A and 1.65 resolution, respectively. YesX crystal belongs to space group P2(1)2(1)2(1) with unit cell parameters of a = 72.9 A, b = 88.1 A, and c = 99.3 A. One molecule is present in an asymmetric unit	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
YesX del_loop	the mutant enzyme shows an endolytic reaction profile. Vmax/Km-value for the mutant enzyme is 275fold lower than the Vmax/KM-value for the wild-type enzyme	Bacillus subtilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	rhamnogalacturonan I	KM-value is 1.78 mg/ml for the wild-type enzyme and 0.56 mg/ml for the, pH 7.5, 30°C	Bacillus subtilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Bacillus subtilis	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
68754	-	x * 68754, calculated from sequence	Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
rhamnogalacturonan I	Bacillus subtilis	responsible for an initial cleavage of the rhamnogalacturonan I region of plant cell wall pectin. Bacillus subtilis strain 168 secretes two rhamnogalacturonan lyases, YesW and YesX, extracellularly. YesW cleaves the glycoside bond of the rhamnogalacturonan chain endolytically, and the resultant oligosaccharides are subsequently converted to disaccharides, unsaturated galacturonyl rhamnose, through the exotype reaction of YesX	2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4,5-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	O31527	-	-

Purification (Commentary)

Purification (Comment)	Organism
native YesX and YesX del_loop mutant	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
rhamnogalacturonan I	responsible for an initial cleavage of the rhamnogalacturonan I region of plant cell wall pectin. Bacillus subtilis strain 168 secretes two rhamnogalacturonan lyases, YesW and YesX, extracellularly. YesW cleaves the glycoside bond of the rhamnogalacturonan chain endolytically, and the resultant oligosaccharides are subsequently converted to disaccharides, unsaturated galacturonyl rhamnose, through the exotype reaction of YesX	Bacillus subtilis	2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4,5-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end	-	?
rhamnogalacturonan I oligosaccharide	exotype eliminative cleavage of alpha-L-rhamnopyranosyl-(1->4)-alpha-D-GalA bonds of rhamnogalacturonan I oligosacharides containing L-rhamnopyranose at the reducing end and 4,5-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end. The products are the disaccharide 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose and the shortened rhamnogalacturonan oligosaccharide containing one 4,5-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end	Bacillus subtilis	2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4,5-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end	-	?

Subunits

Subunits	Comment	Organism
?	x * 68754, calculated from sequence	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
YesX	-	Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Bacillus subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Bacillus subtilis

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Release 2023.1 (January 2023)