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Literature summary for 4.2.2.23 extracted from

  • Mutter, M.; Colquhoun, I.J.
    Schols, H.A.; Beldman, G.; Voragen, A.G.: Rhamnogalacturonase B from Aspergillus aculeatus is a rhamnogalacturonan alpha-L-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronide lyase (1996), Plant Physiol., 110, 73-77.
    View publication on PubMedView publication on EuropePMC
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Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates, no absolute requirement Aspergillus aculeatus

Organism

Organism UniProt Comment Textmining
Aspergillus aculeatus
-
 recombinantly produced in Aspergillus oryzae
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Aspergillus aculeatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity toward polygalacturonic acid at pH 6 or pH 8 either with or without 1 mM Ca2+ in the reaction mixture Aspergillus aculeatus ?
-
 ?
rhamnogalacturonan I rhamnogalacturonase B cleaves alpha-L-Rhap-(1->4)-alpha-D-GalpA bond of rhamnogalacturonan I domains (rhamnogalacturonan backbone) in ramified hairy regions of pectin. Pectin consists of three distinct domains: homogalacturonan (smooth region), rhamnogalacturonan I (RG-I, hairy region), and rhamnogalacturonan-II. Rhamnogalacturonan I consists of repeating stretches of (1->4)-alpha-D-galacturonate-(1->2)-alpha-L-rhamnose dimers in which L-arabinose- and D-galactose-rich side chains may be attached to the rhamnose residues Aspergillus aculeatus rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end eliminative cleavage alpha-L-Rhap-(1->4)-alpha-D-GalpA bond of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving rhamnose at the nonreducing end and unsaturated galacturonic acid at the nonreducing end ?

Synonyms

Synonyms Comment Organism
alpha-L-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronide lyase
-
 Aspergillus aculeatus
RGase B
-
 Aspergillus aculeatus
rhamnogalacturonase B
-
 Aspergillus aculeatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 Mc-Ilvaine buffer Aspergillus aculeatus
8
-
 lyase activity toward saponified modified hairy regions from apple pectin is 20% higher in 20 mM Tris-HCl buffer, pH 8, than in 50 mM NaOAc buffer, pH 6 Aspergillus aculeatus