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Literature summary for 4.2.2.23 extracted from

  • Laatu, M.; Condemine, G.
    Rhamnogalacturonate lyase RhiE is secreted by the out system in Erwinia chrysanthemi (2003), J. Bacteriol., 185, 1642-1649.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Dickeya chrysanthemi

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular RhiE is secreted through the type II Out secretion pathway. RhiE has no disulfide bond. The absence of RhiE secretion in a dsb mutant indicates that disulfide bond formation is required for the biogenesis of the secretion apparatus Dickeya chrysanthemi
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require a divalent cation for its activity. RhiE activity is not increased by the addition of 0.2 mM Ca2+, Mn2+, Co2+, Cu2+, or Zn2+ Dickeya chrysanthemi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61940
-
x * 61940, calculated from sequence Dickeya chrysanthemi
62000
-
x * 62000, SDS-PAGE Dickeya chrysanthemi

Organism

Organism UniProt Comment Textmining
Dickeya chrysanthemi Q8RJP2 RhiE is present in one-third of the strains tested
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Dickeya chrysanthemi 3937 Q8RJP2 RhiE is present in one-third of the strains tested
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the purified enzyme is not able to provoke maceration of potato tubers or chicory leaves. No activity with polygalacturonate, pectin or arabinogalactan Dickeya chrysanthemi ?
-
?
additional information the purified enzyme is not able to provoke maceration of potato tubers or chicory leaves. No activity with polygalacturonate, pectin or arabinogalactan Dickeya chrysanthemi 3937 ?
-
?
rhamnogalacturonan I
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Dickeya chrysanthemi rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end
-
?
rhamnogalacturonan I
-
Dickeya chrysanthemi 3937 rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end
-
?

Subunits

Subunits Comment Organism
? x * 62000, SDS-PAGE Dickeya chrysanthemi
? x * 61940, calculated from sequence Dickeya chrysanthemi

Synonyms

Synonyms Comment Organism
RhiE
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Dickeya chrysanthemi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Dickeya chrysanthemi

Expression

Organism Comment Expression
Dickeya chrysanthemi rhiE expression is strongly induced in the presence of rhamnose but is also regulated by PecT and Crp, two regulators of the transcription of pectinolytic enzyme genes up

General Information

General Information Comment Organism
malfunction reduced virulence of the rhiE mutant indicates that degradation of the RG-I region of pectin is important for full virulence of Erwinia chrysanthemi Dickeya chrysanthemi
physiological function reduced virulence of the rhiE mutant indicates that degradation of the RG-I region of pectin is important for full virulence of Erwinia chrysanthemi Dickeya chrysanthemi