Cloned (Comment) | Organism |
---|---|
- |
Dickeya chrysanthemi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | RhiE is secreted through the type II Out secretion pathway. RhiE has no disulfide bond. The absence of RhiE secretion in a dsb mutant indicates that disulfide bond formation is required for the biogenesis of the secretion apparatus | Dickeya chrysanthemi | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require a divalent cation for its activity. RhiE activity is not increased by the addition of 0.2 mM Ca2+, Mn2+, Co2+, Cu2+, or Zn2+ | Dickeya chrysanthemi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61940 | - |
x * 61940, calculated from sequence | Dickeya chrysanthemi |
62000 | - |
x * 62000, SDS-PAGE | Dickeya chrysanthemi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dickeya chrysanthemi | Q8RJP2 | RhiE is present in one-third of the strains tested | - |
Dickeya chrysanthemi 3937 | Q8RJP2 | RhiE is present in one-third of the strains tested | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the purified enzyme is not able to provoke maceration of potato tubers or chicory leaves. No activity with polygalacturonate, pectin or arabinogalactan | Dickeya chrysanthemi | ? | - |
? | |
additional information | the purified enzyme is not able to provoke maceration of potato tubers or chicory leaves. No activity with polygalacturonate, pectin or arabinogalactan | Dickeya chrysanthemi 3937 | ? | - |
? | |
rhamnogalacturonan I | - |
Dickeya chrysanthemi | rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end | - |
? | |
rhamnogalacturonan I | - |
Dickeya chrysanthemi 3937 | rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62000, SDS-PAGE | Dickeya chrysanthemi |
? | x * 61940, calculated from sequence | Dickeya chrysanthemi |
Synonyms | Comment | Organism |
---|---|---|
RhiE | - |
Dickeya chrysanthemi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Dickeya chrysanthemi |
Organism | Comment | Expression |
---|---|---|
Dickeya chrysanthemi | rhiE expression is strongly induced in the presence of rhamnose but is also regulated by PecT and Crp, two regulators of the transcription of pectinolytic enzyme genes | up |
General Information | Comment | Organism |
---|---|---|
malfunction | reduced virulence of the rhiE mutant indicates that degradation of the RG-I region of pectin is important for full virulence of Erwinia chrysanthemi | Dickeya chrysanthemi |
physiological function | reduced virulence of the rhiE mutant indicates that degradation of the RG-I region of pectin is important for full virulence of Erwinia chrysanthemi | Dickeya chrysanthemi |