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Literature summary for 4.2.2.22 extracted from
- Expression, purification and characterization of pectate lyase A from Aspergillus nidulans in Escherichia coli (2007), World J. Microbiol. Biotechnol., 23, 1057-1064.
Application
| Application | Comment | Organism |
|---|---|---|
| industry | food and textile industry | Aspergillus nidulans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Aspergillus nidulans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | inhibitory | Aspergillus nidulans |  |
| EDTA | - |
Aspergillus nidulans | |
| Zn2+ | inhibitory | Aspergillus nidulans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulating | Aspergillus nidulans | |
| Fe2+ | stimulating | Aspergillus nidulans | |
| Fe3+ | stimulating | Aspergillus nidulans | |
| Mg2+ | stimulating | Aspergillus nidulans | |
| Mn2+ | stimulating | Aspergillus nidulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
SDS-PAGE, Sephadex G-100 chromatography | Aspergillus nidulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
GR5 | - |
| Aspergillus nidulans GR5 | - |
GR5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| the recombinant protein using Ni2+-nitrilotriacetateagarose column chromatography | Aspergillus nidulans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus nidulans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.37 | - |
90% esterified pectin | Aspergillus nidulans |
| 6.38 | - |
60% esterified pectin | Aspergillus nidulans |
| 57.3 | - |
polygalacturonic acid | Aspergillus nidulans |
| 63.7 | - |
30% esterified pectin | Aspergillus nidulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin | low-esterified pectin is the optimum substrate for the PelA, higher-esterified pectin is hardly cleaved | Aspergillus nidulans | ? | - |
? | |
| pectin | low-esterified pectin is the optimum substrate for the PelA, higher-esterified pectin is hardly cleaved | Aspergillus nidulans GR5 | ? | - |
? | |
| polygalacturonic acid | - |
Aspergillus nidulans | 4,5-unsaturated polygalacturonic acid | - |
? | |
| polygalacturonic acid | - |
Aspergillus nidulans GR5 | 4,5-unsaturated polygalacturonic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exopectate lyase | - |
Aspergillus nidulans |
| pectate lyase A | - |
Aspergillus nidulans |
| pectate transeliminase | - |
Aspergillus nidulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus nidulans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 60 | - |
Aspergillus nidulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 55 | - |
Aspergillus nidulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Aspergillus nidulans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | - |
Aspergillus nidulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | at 50°C | Aspergillus nidulans |
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