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Literature summary for 4.2.2.20 extracted from

  • Lee, H.; McKeon, R.J.; Bellamkonda, R.V.
    Sustained delivery of thermostabilized chABC enhances axonal sprouting and functional recovery after spinal cord injury (2010), Proc. Natl. Acad. Sci. USA, 107, 3340-3345.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine development of thermostabilized chondroitinase ABC and a system for its sustained local delivery in vivo, obviating the need for chronically implanted catheters and pumps. Presence of with 1M trehalose significantly enhances chondroitinase ABC thermal stability and prolongs enzyme activity. Thermostabilized chondroitinase ABC remains active at 37°C in vitro for up to 4 weeks. Chondroitin sulfate proteoglycan levels remain low in vivo up to 6 weeks post-spinal cord injury when thermostabilized chondroitin sulfate proteoglycan ABC is delivered by a hydrogel-microtube scaffold system. Axonal growth and functional recovery following the sustained local release of thermostabilized chondroitinase ABC versus a single treatment of unstabilized chondroitinase ABC demonstrate significant differences in chondroitin sulfate proteoglycan digestion. Animals treated with thermostabilized chondroitinase ABC in combination with sustained neurotrophin-3 delivery show significant improvement in locomotor function and enhanced growth of cholera toxin B subunit-positive sensory axons and sprouting of serotonergic fibers Proteus vulgaris

Organism

Organism UniProt Comment Textmining
Proteus vulgaris
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Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
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Proteus vulgaris
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