Literature summary for 4.2.2.2 extracted from

Alahuhta, M.; Chandrayan, P.; Kataeva, I.; Adams, M.W.; Himmel, M.E.; Lunin, V.V.
A 1.5 A resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase (2011), Acta Crystallogr. Sect. F, 67, 1498-1500.

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)	Caldicellulosiruptor bescii

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant N-terminally His6-tagged enzyme, sitting drop vapor diffusion method, mixing of 0.0002 ml of protein containing 15 mg/ml protein, 20 mM acetic acid, pH 5, 100 mM NaCl and 5 mM CaCl2, with 0.0002 ml of well solution, containing 0.2 M ammonium phosphate monobasic, 0.1 M Tris, pH 8.5, and 50% v/v 2-methyl-2,4-pentanediol, 20°C, X-ray diffraction structure determination and analysis at 1.5 A resolution, molecular replacement method, modeling	Caldicellulosiruptor bescii

Crystallization (Comment)

Organism

purified recombinant N-terminally His6-tagged enzyme, sitting drop vapor diffusion method, mixing of 0.0002 ml of protein containing 15 mg/ml protein, 20 mM acetic acid, pH 5, 100 mM NaCl and 5 mM CaCl2, with 0.0002 ml of well solution, containing 0.2 M ammonium phosphate monobasic, 0.1 M Tris, pH 8.5, and 50% v/v 2-methyl-2,4-pentanediol, 20°C, X-ray diffraction structure determination and analysis at 1.5 A resolution, molecular replacement method, modeling

Caldicellulosiruptor bescii

Organism

Organism	UniProt	Comment	Textmining
Caldicellulosiruptor bescii	B9MKT4	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, gel filtration, and ultrafiltration	Caldicellulosiruptor bescii

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to pectate lyase family 3	Caldicellulosiruptor bescii

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Release 2023.1 (January 2023)