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Literature summary for 4.2.2.2 extracted from

  • Zhao, Q.; Yuan, S.; Zhang, Y.; Zhu, H.; Dai, C.; Yang, F.; Han, F.
    Expression, purification and characterization of pectate lyase A from Aspergillus nidulans in Escherichia coli (2007), World J. Microbiol. Biotechnol., 23, 1057-1064.
    View publication on PubMed
Search for more literature for 4.2.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aspergillus nidulans

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 91% inhibition Aspergillus nidulans
EDTA 1 mM, completely represses PelA enzyme activity Aspergillus nidulans
Zn2+ 1 mM, 40% inhibition Aspergillus nidulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km of 0.50 mg/ml for polygalacturonic acid Aspergillus nidulans

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ 1 mM, stimulates activity up to 120% Aspergillus nidulans
Ca2+ 1 mM, activity is enhanced by 1209% Aspergillus nidulans
Fe2+ 1 mM, stimulates activity up to 592% Aspergillus nidulans
Fe3+ 1 mM, stimulates activity up to 348% Aspergillus nidulans
Mg2+ 1 mM, stimulates activity up to 368% Aspergillus nidulans
Mn2+ 1 mM, activity is enhanced by 1221% Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Aspergillus nidulans

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PelA efficiently macerates mung bean hypocotyls and potato tuber tissues into single cells Aspergillus nidulans ?
-
 ?
additional information PelA efficiently macerates mung bean hypocotyls and potato tuber tissues into single cells Aspergillus nidulans GR5 ?
-
 ?
pectin low-esterified pectin (30%) is the optimum substrate for the PelA, higher-esterified pectin is hardly cleaved Aspergillus nidulans ?
-
 ?
pectin low-esterified pectin (30%) is the optimum substrate for the PelA, higher-esterified pectin is hardly cleaved Aspergillus nidulans GR5 ?
-
 ?
polygalacturonic acid
-
 Aspergillus nidulans ?
-
 ?
polygalacturonic acid
-
 Aspergillus nidulans GR5 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Aspergillus nidulans

Synonyms

Synonyms Comment Organism
pectate lyase A
-
 Aspergillus nidulans
PelA
-
 Aspergillus nidulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus nidulans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 about 50% of maximal activity at 30°C and at 70°C Aspergillus nidulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life: 1 h Aspergillus nidulans
60
-
 readily inactivated above Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9.5
-
 Aspergillus nidulans

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 pH 7.5: about 50% of maximal activity, pH 10: about 50% of maximal activity Aspergillus nidulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 40°C, 1 h, stable Aspergillus nidulans