Literature summary for 4.2.2.2 extracted from

Liao, C.H.; Fett, W.; Tzean, S.S.; Hoffman, G.
Detection and sequence analysis of an altered pectate lyase gene in Pseudomonas syringae pv. glycinea and related bacteria (2006), Can. J. Microbiol., 52, 1051-1059.

Cloned(Commentary)

Cloned (Comment)	Organism
none of the 10 Pseudomonas syringae pv. glycinea strains examined exhibits pectolytic activity. The pectate lyase gene is detected in four out of four strains examined. The pel gene contains a single-base insertion, a double-base insertion, and an 18-bp deletion, which can lead to the synthesis of an inactive pectate lyase protein. The function of the protein can be restored by removing the unwanted base insertion and by filling in the 18-bp deletions by site-directed mutagenesis and expression in Escherichia coli	Pseudomonas syringae

Cloned (Comment)

Organism

none of the 10 Pseudomonas syringae pv. glycinea strains examined exhibits pectolytic activity. The pectate lyase gene is detected in four out of four strains examined. The pel gene contains a single-base insertion, a double-base insertion, and an 18-bp deletion, which can lead to the synthesis of an inactive pectate lyase protein. The function of the protein can be restored by removing the unwanted base insertion and by filling in the 18-bp deletions by site-directed mutagenesis and expression in Escherichia coli

Pseudomonas syringae

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas syringae	-	pv. glycinea	-

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Release 2023.1 (January 2023)