General Stability | Organism |
---|---|
enzyme from Alpine strain A15 is stable up to fourfold freezing and thawing | Mrakia frigida |
enzyme from Siberian strain AG25 is stable up to fourfold freezing and thawing | Mrakia frigida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, 12% loss of activity | Bacillus subtilis | |
EDTA | 1 mM, enzyme from Alpine strain A15 loses 67% of initial activity, complete inactivation at 10 mM; 1 mM, enzyme from Siberian strain AG25 loses 85% of initial activity, complete inactivation at 10 mM | Mrakia frigida | |
SDS | 1%, strong inhibition | Bacillus subtilis | |
SDS | strong inhibition of enzyme from Alpine strain A15; strong inhibition of enzyme from Siberian strain AG25 | Mrakia frigida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
extracellular | - |
Mrakia frigida | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 71% of maximal activity in absence of Ca2+ | Bacillus subtilis | |
Ca2+ | enzyme from Alpine strain A15 completely loses activity in absence of Ca2+ | Mrakia frigida | |
Ca2+ | enzyme from Siberian strain AG25 completely loses activity in absence of Ca2+ | Mrakia frigida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37500 | - |
x * 37500, enzyme from Alpine strain A15, SDS-PAGE | Mrakia frigida |
38000 | - |
x * 38000, Siberian strain AG25, SDS-PAGE | Mrakia frigida |
38500 | - |
x * 38500, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Mrakia frigida | - |
Alpine strain A15 | - |
Mrakia frigida | - |
Siberian strain AG25 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Bacillus subtilis | - |
culture medium | enzyme is detected in the late logarithmic growth phase, enzyme production is highest in the early stationary growth phase. The Alpine strain A15 produces high amounts of enzyme only at a cultivation temperature of 1-10°C, with a maximum of enzyme production at 5°C. Enzyme production by the Siberian strain AG25 is approximately twice as high than production by the Alpine strain A15 | Mrakia frigida | - |
culture medium | enzyme is detected in the late logarithmic growth phase, enzyme production is highest in the early stationary growth phase. The Siberian strain AG25 produces the enzyme over the growth temperature range of 1-20°C, with a maximum of enzyme production at 1°C. Enzyme production by the Siberian strain AG25 is approximately twice as high than production by the Alpine strain A15 | Mrakia frigida | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 38500, SDS-PAGE | Bacillus subtilis |
? | x * 37500, enzyme from Alpine strain A15, SDS-PAGE | Mrakia frigida |
? | x * 38000, Siberian strain AG25, SDS-PAGE | Mrakia frigida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
enzyme from Alpine strain A15 | Mrakia frigida |
30 | - |
enzyme from Siberian strain AG25 | Mrakia frigida |
60 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 40 | 15°C: about 40% of maximal activity, enzyme from Alpine strain A15, 35°C: enzyme from Alpine strain shows about 80% of maximal activity, 40°C: enzyme from Alpine strain shows about 55% of maximal activity | Mrakia frigida |
15 | 40 | 15°C: enzyme from Siberian strain AG25 shows about 40% of maximal activity, 35°C: enzyme from Siberian strain AG25 shows about 45% of maximal activity, 40°C: enzyme from Siberian strain AG25 shows about 10% of maximal activity | Mrakia frigida |
40 | 65 | 40°C: about 40% of maximal activity, 65°C: about 65% of maximal activity | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
2 | - |
48 h, enzyme from Alpine strain A15 loses 21% of initial activity | Mrakia frigida |
2 | - |
48 h, enzyme from Siberian strain AG25 loses 5% of initial activity | Mrakia frigida |
30 | - |
pH 9.0, 15 min, enzyme from Alpine strain A15 loses 40% of initial activity | Mrakia frigida |
30 | - |
pH 9.0, 15 min, enzyme from Siberian strain AG25 loses 35% of initial activity | Mrakia frigida |
40 | - |
pH 9.0, 15 min, enzyme from Alpine strain A15 loses 10% of initial activity | Mrakia frigida |
40 | - |
pH 9.0, 15 min, enzyme from Siberian strain AG25 loses 95% of initial activity | Mrakia frigida |
60 | - |
pH 9.0, 15 min, about 20% loss of activity | Bacillus subtilis |
70 | - |
pH 9.0, 15 min, 50% loss of activity | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Bacillus subtilis |
8.5 | - |
enzyme from Siberian strain AG25 | Mrakia frigida |
9 | - |
enzyme from Alpine strain A15 | Mrakia frigida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | pH 7.0: about 70% of maximal activity, pH 9.0: about 65% of maximal activity | Bacillus subtilis |
7.5 | 9.5 | pH 7.5: enzyme from Alpine strain A15 shows about 40% of maximal activity, pH 9.5: enzyme from Alpine strain A15 shows about 60% of maximal activity | Mrakia frigida |
7.5 | 9.5 | pH 7.5: enzyme from Siberian strain AG25 shows about 40% of maximal activity, pH 9.0: enzyme from Siberian strain AG25 shows about 75% of maximal activity, pH 9.5: enzyme from Siberian strain AG25 shows about 25% of maximal activity | Mrakia frigida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | 1 h, 35-40% loss of activity, enzyme from Alpine strain A15 and Siberian strain AG25 | Mrakia frigida |
9 | 10 | 1 h, 35-40% loss of activity, enzyme from Siberian strain AG25 | Mrakia frigida |