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Literature summary for 4.2.2.2 extracted from
- Purification and characterization of a new bioscouring pectate lyase from Bacillus pumilus BK2 (2006), J. Biotechnol., 121, 390-401.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | the enzyme can be used for bioscouring of cotton | Bacillus pumilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 0.38 mM, 50% inhibition | Bacillus pumilus |  |
| EDTA | 0.09 mM, 50% inhibition | Bacillus pumilus | |
| Zn2+ | 2.8 mM, 50% inhibition | Bacillus pumilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | KM-value for polygalacturonate is 0.24 g/l, at pH 7.8, 50°C. KM-value is 0.24 g/l for not esterified pectin, 0.36 g/l for pectin with 5.3% esterification, 0.35 g/l for pectin with 29.5% esterification, 1.34 g/l for pectin with 51.4% esterification | Bacillus pumilus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus pumilus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme does not require Ca2+ | Bacillus pumilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37300 | - |
x * 37300, SDS-PAGE | Bacillus pumilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus pumilus | - |
- |
- |
| Bacillus pumilus BK2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus pumilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| esterified pectin | affinity shows a maximum for intermediate esterified pectins and decreases over a value of 50% of esterification. The best substrate is 29.5% methylated pectin | Bacillus pumilus | unsaturated tetragalacturonic acid | - |
? | |
| esterified pectin | affinity shows a maximum for intermediate esterified pectins and decreases over a value of 50% of esterification. The best substrate is 29.5% methylated pectin | Bacillus pumilus BK2 | unsaturated tetragalacturonic acid | - |
? | |
| polygalacturonate | the enzyme cleaves polygalacturonic acid via a beta-elimination mechanism | Bacillus pumilus | unsaturated tetragalacturonic acid | - |
? | |
| polygalacturonate | the enzyme cleaves polygalacturonic acid via a beta-elimination mechanism | Bacillus pumilus BK2 | unsaturated tetragalacturonic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37300, SDS-PAGE | Bacillus pumilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Bacillus pumilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
half-life: more than 75 h | Bacillus pumilus |
| 80 | - |
half-life at pH 8.0 and 7.0 is 1.2 min, half-life at pH 9.0 is 0.8 min | Bacillus pumilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Bacillus pumilus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus pumilus | 2D PAGE, pH-range 3-10 | - |
8.5 |
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