Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Treponema pectinovorum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 3 mM, complete inhibition. Lyase activity can be restored by further addition of 5 mM CaCl2 | Treponema pectinovorum | |
MnSO4 | 0.1 mM, 10% decrease in activity | Treponema pectinovorum | |
ZnSO4 | 0.1 mM, 90% decrease in activity | Treponema pectinovorum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | requires Ca2+, optimal activity with 0.25 mM | Treponema pectinovorum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Treponema pectinovorum | Q7X0P2 | ATCC 33768 | - |
Purification (Comment) | Organism |
---|---|
6*His-tagged recombinant enzyme | Treponema pectinovorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
esterified pectin | 94% esterified pectin. 97% of the activity with polygalacturonic acid | Treponema pectinovorum | ? | - |
? | |
pectin | 80% of the activity with polygalacturonic acid | Treponema pectinovorum | ? | - |
? | |
polygalacturonic acid | - |
Treponema pectinovorum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PelA | - |
Treponema pectinovorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Treponema pectinovorum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 50 | 37°C: about 85% of maximal activity, 50°C: about 75% of maximal activity | Treponema pectinovorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Treponema pectinovorum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | pH 8.0: about 50% of maximal activity, pH 10.0: about 40% of maximal activity | Treponema pectinovorum |