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Literature summary for 4.2.2.2 extracted from
- Biochemical characterization of the pectate lyase PelZ of Erwinia chrysanthemi 3937 (1998), Biochim. Biophys. Acta, 1383, 188-196.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overproduced in Escherichia coli | Dickeya chrysanthemi |
General Stability
| General Stability | Organism |
|---|---|
| Ca2+, Mn2+ or polygalacturonate increase thermostability | Dickeya chrysanthemi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Dickeya chrysanthemi |  |
| epicatechin | - |
Dickeya chrysanthemi | |
| iodoacetic acid | - |
Dickeya chrysanthemi | |
| polygalacturonic acid | at concentrations exceeding the Km-value | Dickeya chrysanthemi | |
| salicylic acid | - |
Dickeya chrysanthemi | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | isoenzyme PelZ | Dickeya chrysanthemi | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | optimal concentration: 0.2 mM | Dickeya chrysanthemi | |
| Mn2+ | optimal concentration: 0.1 mM | Dickeya chrysanthemi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, isoenzyme PelZ, SDS-PAGE | Dickeya chrysanthemi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dickeya chrysanthemi | - |
3937 | - |
| Dickeya chrysanthemi | - |
at least 7 isoenzymes, 5 major isoenzymes, PelA, PelB, PelC, PelD, Pel E and two minor isoenzyme PelL and PelZ | - |
| Dickeya chrysanthemi 3937 | - |
3937 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Dickeya chrysanthemi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin | with a low degree of methylation | Dickeya chrysanthemi | ? | - |
? | |
| pectin | with a low degree of methylation | Dickeya chrysanthemi 3937 | ? | - |
? | |
| polygalacturonic acid | with a low degree of methylation | Dickeya chrysanthemi | unsaturated oligogalacturonate | - |
? | |
| polygalacturonic acid | with a low degree of methylation | Dickeya chrysanthemi 3937 | unsaturated oligogalacturonate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, isoenzyme PelZ, SDS-PAGE | Dickeya chrysanthemi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
10 min, 50% loss of activity in absence of cations, 10% loss of activity in presence of Mn2+, Ca2+ or polygalacturonic acid | Dickeya chrysanthemi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Dickeya chrysanthemi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | pH 7: less than 10% of maximal activity, pH 8.5-9: optimum | Dickeya chrysanthemi |
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