Literature summary for 4.2.2.2 extracted from

Sugiura, J.; Yasuda, M.; Kamimiya, S.; Izaki, K.; Takahashi, H.
Purification and properties of two pectate lyases produced by Erwinia carotovora (1984), J. Gen. Appl. Microbiol., 30, 167-175.

No PubMed abstract available

Search for more literature for 4.2.2.2

Inhibitors

Inhibitors	Comment	Organism	Structure
Cd2+	-	Pectobacterium carotovorum
Cu2+	-	Pectobacterium carotovorum
Mg2+	-	Pectobacterium carotovorum
Mn2+	-	Pectobacterium carotovorum
Ni2+	-	Pectobacterium carotovorum
Zn2+	-	Pectobacterium carotovorum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Pectobacterium carotovorum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	0.5 mM, 7fold activation of pectate lyase I, 4fold activation of pectate lyase II	Pectobacterium carotovorum

Organism

Organism	UniProt	Comment	Textmining
Pectobacterium carotovorum	-	pectate lyase I and pectate lyase II	-
Pectobacterium carotovorum	-	formerly Erwinia aroideae	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
side-chain modification	neutral sugar content : 2.5% for pectate lyase I and 4.8% for pectate lyase II	Pectobacterium carotovorum

Purification (Commentary)

Purification (Comment)	Organism
pectate lyase I and pectate lyase II	Pectobacterium carotovorum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	pectate lyase I	Pectobacterium carotovorum
60	-	pectate lyase II	Pectobacterium carotovorum

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	65	30°C: about 45% of maximal activity, 65°C: about 40% of maximal activity, pectate lyase I	Pectobacterium carotovorum
40	70	40°C: about 40% of maximal activity, 70°C: about 30% of maximal activity	Pectobacterium carotovorum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
10	-	pectate lyase I and pectate lyase II	Pectobacterium carotovorum

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Release 2023.1 (January 2023)