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Literature summary for 4.2.2.19 extracted from

  • Pojasek, K.; Raman, R.; Kiley, P.; Venkataraman, G.; Sasisekharan, R.
    Biochemical characterization of the chondroitinase B active site (2002), J. Biol. Chem., 277, 31179-31186.
    View publication on PubMed
Search for more literature for 4.2.2.19

Protein Variants

Protein Variants Comment Organism
E333A PCR site-directed mutagenesis, E333 is a key residue in catalysis, reduced activity Pedobacter heparinus
H272A PCR site-directed mutagenesis, H272 is a key residue in catalysis, reduced activity Pedobacter heparinus
K250A PCR site-directed mutagenesis, inactive mutant Pedobacter heparinus
R271A PCR site-directed mutagenesis, R271 is a key residue in catalysis Pedobacter heparinus
R363A PCR site-directed mutagenesis, R363 is a key residue in catalysis, 2fold increased activity Pedobacter heparinus
R364A PCR site-directed mutagenesis, highly reduced activity, altered product profil, residue is involved in determination of substrate specificity Pedobacter heparinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of wild-type and mutant enzymes Pedobacter heparinus
0.0027
-
 dermatan sulfate mutant H272A, pH 8.0, 30°C Pedobacter heparinus
0.0028
-
 dermatan sulfate mutant E333A, pH 8.0, 30°C Pedobacter heparinus
0.0046
-
 dermatan sulfate mutant R363A, pH 8.0, 30°C Pedobacter heparinus
0.0046
-
 dermatan sulfate wild-type enzyme, pH 8.0, 30°C Pedobacter heparinus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no bound Ca2+ Pedobacter heparinus

Organism

Organism UniProt Comment Textmining
Pedobacter heparinus Q46079
-
-

Reaction

Reaction Comment Organism Reaction ID
[dermatan sulfate]n = [dermatan sulfate]n-1 + 4-deoxy-beta-D-gluc-4-enuronosyl-1,3-GalNAc acts on dermatan sulfate as sole substrate, active site structure involving Lys250, Arg271, His272, and Glu333, substrate binding mechanism involving Arg363 and Arg364 Pedobacter heparinus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dermatan sulfate
-
 Pedobacter heparinus ?
-
 ?
dermatan sulfate analysis of interactions between enzyme and substrate Pedobacter heparinus ?
-
 ?

Subunits

Subunits Comment Organism
More structural analysis Pedobacter heparinus

Synonyms

Synonyms Comment Organism
chondroitinase B
-
 Pedobacter heparinus
lyase, chondroitin B
-
 Pedobacter heparinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pedobacter heparinus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.6
-
 dermatan sulfate mutant E333A, pH 8.0, 30°C Pedobacter heparinus
29
-
 dermatan sulfate mutant H272A, pH 8.0, 30°C Pedobacter heparinus
190
-
 dermatan sulfate wild-type enzyme, pH 8.0, 30°C Pedobacter heparinus
404
-
 dermatan sulfate mutant R363A, pH 8.0, 30°C Pedobacter heparinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pedobacter heparinus