Protein Variants | Comment | Organism |
---|---|---|
E333A | PCR site-directed mutagenesis, E333 is a key residue in catalysis, reduced activity | Pedobacter heparinus |
H272A | PCR site-directed mutagenesis, H272 is a key residue in catalysis, reduced activity | Pedobacter heparinus |
K250A | PCR site-directed mutagenesis, inactive mutant | Pedobacter heparinus |
R271A | PCR site-directed mutagenesis, R271 is a key residue in catalysis | Pedobacter heparinus |
R363A | PCR site-directed mutagenesis, R363 is a key residue in catalysis, 2fold increased activity | Pedobacter heparinus |
R364A | PCR site-directed mutagenesis, highly reduced activity, altered product profil, residue is involved in determination of substrate specificity | Pedobacter heparinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wild-type and mutant enzymes | Pedobacter heparinus | |
0.0027 | - |
dermatan sulfate | mutant H272A, pH 8.0, 30°C | Pedobacter heparinus | |
0.0028 | - |
dermatan sulfate | mutant E333A, pH 8.0, 30°C | Pedobacter heparinus | |
0.0046 | - |
dermatan sulfate | mutant R363A, pH 8.0, 30°C | Pedobacter heparinus | |
0.0046 | - |
dermatan sulfate | wild-type enzyme, pH 8.0, 30°C | Pedobacter heparinus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no bound Ca2+ | Pedobacter heparinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pedobacter heparinus | Q46079 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[dermatan sulfate]n = [dermatan sulfate]n-1 + 4-deoxy-beta-D-gluc-4-enuronosyl-1,3-GalNAc | acts on dermatan sulfate as sole substrate, active site structure involving Lys250, Arg271, His272, and Glu333, substrate binding mechanism involving Arg363 and Arg364 | Pedobacter heparinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dermatan sulfate | - |
Pedobacter heparinus | ? | - |
? | |
dermatan sulfate | analysis of interactions between enzyme and substrate | Pedobacter heparinus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structural analysis | Pedobacter heparinus |
Synonyms | Comment | Organism |
---|---|---|
chondroitinase B | - |
Pedobacter heparinus |
lyase, chondroitin B | - |
Pedobacter heparinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pedobacter heparinus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
dermatan sulfate | mutant E333A, pH 8.0, 30°C | Pedobacter heparinus | |
29 | - |
dermatan sulfate | mutant H272A, pH 8.0, 30°C | Pedobacter heparinus | |
190 | - |
dermatan sulfate | wild-type enzyme, pH 8.0, 30°C | Pedobacter heparinus | |
404 | - |
dermatan sulfate | mutant R363A, pH 8.0, 30°C | Pedobacter heparinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pedobacter heparinus |