Literature summary for 4.2.2.19 extracted from

Tkalec, A.L.; Fink, D.; Blain, F.; Zhang-Sun, G.; Laliberte, M.; Bennett, D.C.; Gu, K.; Zimmermann, J.J.F.; Su, H.
Isolation and expression in Escherichia coli of cslA and cslB, genes coding for the chondroitin sulfate-degrading enzymes chondroitinase AC and chondroitinase B, respectively, from Flavobacterium heparinum (2000), Appl. Environ. Microbiol., 66, 29-35.

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Activating Compound

Activating Compound	Comment	Organism	Structure
chondroitin sulfate A	induces enzyme expression in cells	Pedobacter heparinus

Cloned(Commentary)

Cloned (Comment)	Organism
gene cslB, expression in Escherichia coli without the N-terminal signal sequence, different expression plasmids constructed, expression in soluble and insoluble form	Pedobacter heparinus

Inhibitors

Inhibitors	Comment	Organism	Structure
glucose	represses enzyme induction in cells by chondroitin sulfate A	Pedobacter heparinus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	x * 55000, SDS-PAGE, recombinant and native enzyme	Pedobacter heparinus

Organism

Organism	UniProt	Comment	Textmining
Pedobacter heparinus	Q46079	i.e. Cytophaga heparina, Sphingobacterium heparinum, or Pedobacter heparinus, gene cslB	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dermatan sulfate	-	Pedobacter heparinus	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 55000, SDS-PAGE, recombinant and native enzyme	Pedobacter heparinus

Synonyms

Synonyms	Comment	Organism
ChnB	-	Pedobacter heparinus
chondroitinase B	-	Pedobacter heparinus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	recombinant and native enzyme are completely stable	Pedobacter heparinus

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Release 2023.1 (January 2023)