Literature summary for 4.2.2.19 extracted from

Li, Y.; Matte, A.; Su, H.; Cygler, M.
Crystallization and preliminary X-ray analysis of chondroitinase B from Flavobacterium heparinum (1999), Acta Crystallogr. Sect. D, 55, 1055-1057.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of enzyme, residues 25-506, in Flavobacterium heparinum cells	Pedobacter heparinus

Crystallization (Commentary)

Crystallization (Comment)	Organism
6-8 mg/ml purified recombinant enzyme, residues 25-506, in 20 mM Tris-HCl, pH 8.0, 1 mM sodium phosphate, pH 7.0, 3 mM NaCl, 0.5 mM phenylmethylsulfonyl fluoride, 0.001 mg/ml aprotinin, 0.001 mg/ml leupeptin, 0.001 mg/ml E64, hanging drop vapour diffusion method, 292 K, mixed with a double volume of reservoir solution containing 19% w/v PEG 8000, 100 mM bicine buffer, pH 9.0, or 100 mM Tris-HCl, pH 8.8, 0.15 M ammonium acetate, 15% v/v 2-methyl-2,4-pentanediol, drops are suspended over 1 ml of resevroir solution, crystals appear overnight, seeds are introduced into drops conisting of 0.002 ml protein solution, 6.1 mg/ml protein, and 0.004 ml reservoir solution containing 16.5% w/v PEG 8000, 0.1 M Tris-HCl, pH 8.8, 15% v/v 2-methyl-2,4-pentanediol, 0.25 M ammonium acetate, 292 K, 2-3 weeks, X-ray diffraction structure determination and analysis at 2.20-2.28 A resolution	Pedobacter heparinus

Crystallization (Comment)

Organism

6-8 mg/ml purified recombinant enzyme, residues 25-506, in 20 mM Tris-HCl, pH 8.0, 1 mM sodium phosphate, pH 7.0, 3 mM NaCl, 0.5 mM phenylmethylsulfonyl fluoride, 0.001 mg/ml aprotinin, 0.001 mg/ml leupeptin, 0.001 mg/ml E64, hanging drop vapour diffusion method, 292 K, mixed with a double volume of reservoir solution containing 19% w/v PEG 8000, 100 mM bicine buffer, pH 9.0, or 100 mM Tris-HCl, pH 8.8, 0.15 M ammonium acetate, 15% v/v 2-methyl-2,4-pentanediol, drops are suspended over 1 ml of resevroir solution, crystals appear overnight, seeds are introduced into drops conisting of 0.002 ml protein solution, 6.1 mg/ml protein, and 0.004 ml reservoir solution containing 16.5% w/v PEG 8000, 0.1 M Tris-HCl, pH 8.8, 15% v/v 2-methyl-2,4-pentanediol, 0.25 M ammonium acetate, 292 K, 2-3 weeks, X-ray diffraction structure determination and analysis at 2.20-2.28 A resolution

Pedobacter heparinus

General Stability

General Stability	Organism
protease inhibitors like aprotinin, leupeptin or E64 are required for stability of the enzyme during storage at 277 K	Pedobacter heparinus

Organism

Organism	UniProt	Comment	Textmining
Pedobacter heparinus	Q46079	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme comprising residues 25-506 overexpressed in Flavobacterium heparinum cells, to homogeneity	Pedobacter heparinus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dermatan sulfate	-	Pedobacter heparinus	unsaturated dermatan sulfate oligosaccharide	-	?

Synonyms

Synonyms	Comment	Organism
chondroitinase B	-	Pedobacter heparinus