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Literature summary for 4.2.2.18 extracted from

  • Kim, H.Y.; Kim, I.H.; Choi, Y.J.
    An efficient purification with a high recovery of the inulin fructotransferase of Arthrobacter sp. A-6 from recombinant Escherichia coli (2000), Biotechnol. Lett., 22, 291-293.
No PubMed abstract available
Search for more literature for 4.2.2.18

Cloned(Commentary)

Cloned (Comment) Organism
strain A-6, functional expression in Escherichia coli DH5alpha/pDFE Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46500
-
 x * 46500, SDS-PAGE Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. Q9FA01
-
-
Arthrobacter sp. A-6 Q9FA01
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme from strain A-6 recombinant in Escherichia coli Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
740
-
 purified recombinant enzyme Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin
-
 Arthrobacter sp. 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? i.e. DFA III ?
inulin
-
 Arthrobacter sp. A-6 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? i.e. DFA III ?

Subunits

Subunits Comment Organism
? x * 46500, SDS-PAGE Arthrobacter sp.

Synonyms

Synonyms Comment Organism
IFTase
-
 Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 recombinant enzyme Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 recombinant enzyme, stable for 5 h Arthrobacter sp.
80
-
 recombinant enzyme, 65% remaining activity after 30 min Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Arthrobacter sp.