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Literature summary for 4.2.2.17 extracted from
- Purification of inulin fructotransferase (DFA I-producing) from Arthrobacter sp. MCI2493 and production of DFA I from inulin by the enzyme (1994), Biosci. Biotechnol. Biochem., 58, 574-575.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | di-D-fructofuranose-1,2':2,1'-dianhydride is expected to be useful as a low-calorie sweetener | Arthrobacter |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
SDS-PAGE and gel filtration | Arthrobacter |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin | Arthrobacter | - |
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ? | i.e. DFA I | ? |  |
| inulin | Arthrobacter MC12493 | - |
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ? | i.e. DFA I | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter | - |
MC12493 | - |
| Arthrobacter MC12493 | - |
MC12493 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin | - |
Arthrobacter | 3 di-D-fructofuranose-1,2':2,1'-dianhydride + ? | i.e. DFA I | ? | |
| inulin | - |
Arthrobacter MC12493 | 3 di-D-fructofuranose-1,2':2,1'-dianhydride + ? | i.e. DFA I | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 40000, gel filtration | Arthrobacter |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Arthrobacter |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
pH 6, 30 min., 80% activity | Arthrobacter |
| 80 | - |
30 min., complete activity loss | Arthrobacter |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Arthrobacter |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
30°C, 30 min., stable | Arthrobacter |
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