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Literature summary for 4.2.2.16 extracted from

  • Yang, S.J.; Park, N.H.; Lee, T.H.; Cha, J.
    Expression, purification and characterization of a recombinant levan fructotransferase (2002), Biotechnol. Appl. Biochem., 35, 199-203.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
subcloned into a high-expression vector, pET-29b, and the recombinant enzyme with a tag of six histidine is overexpressed in Escherichia coli Microbacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km for levan: 2 mg/ml at pH 7.0 and 40°C Microbacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Microbacterium sp. 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
x * 54000, SDS-PAGE Microbacterium sp.

Organism

Organism UniProt Comment Textmining
Microbacterium sp. Q9EVQ9 AL-210
-
Microbacterium sp. AL-210 Q9EVQ9 AL-210
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Microbacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
145.2
-
-
Microbacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
levan
-
Microbacterium sp. 5 di-D-fructose-2,6':2',6 dianhydride + ?
-
?
levan
-
Microbacterium sp. AL-210 5 di-D-fructose-2,6':2',6 dianhydride + ?
-
?
additional information the enzyme never hydrolyzes beta-2,1-fructosyl-linked fructans, such as inulin, as well as oligosaccharides such as 1-kestose and nystose Microbacterium sp. ?
-
?
additional information the enzyme never hydrolyzes beta-2,1-fructosyl-linked fructans, such as inulin, as well as oligosaccharides such as 1-kestose and nystose Microbacterium sp. AL-210 ?
-
?

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE Microbacterium sp.

Synonyms

Synonyms Comment Organism
LftM
-
Microbacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
Microbacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9 about 50% of maximal activity at pH 4.5 and pH 9.0 Microbacterium sp.