Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.2.16 extracted from

  • Jang, K.H.; Ryu, E.J.; Park, B.S.; Song, K.B.; Kang, S.A.; Kim, C.H.; Uhm, T.B.; Park, Y.I.; Rhee, S.K.
    Levan fructotransferase from Arthrobacter oxydans, J17-21 catalyzes the formation of the di-D-fructose dianhydride IV from levan (2003), J. Agric. Food Chem., 51, 2632-2636.
    View publication on PubMed
Search for more literature for 4.2.2.16

Inhibitors

Inhibitors Comment Organism Structure
Ag2SO4 1 mM, complete inhibition Pseudarthrobacter oxydans
CoCl2 1 mM, 16% inhibition Pseudarthrobacter oxydans
CuSO4 1 mM, 36% inhibition Pseudarthrobacter oxydans
FeSO4 1 mM, 99% inhibition Pseudarthrobacter oxydans
HgSO4 1 mM, 84% inhibition Pseudarthrobacter oxydans
MnCl2 1 mM, 44% inhibition Pseudarthrobacter oxydans
NaCl 1 mM, 10% inhibition Pseudarthrobacter oxydans
NiCl2 1 mM, 29% inhibition Pseudarthrobacter oxydans

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 1 mM, activation to 136% of control Pseudarthrobacter oxydans

Organism

Organism UniProt Comment Textmining
Pseudarthrobacter oxydans
-
-
-
Pseudarthrobacter oxydans J17-21
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudarthrobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1432
-
-
 Pseudarthrobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
levan levan from Zymomonas mobilis, conversion yield is 35% Pseudarthrobacter oxydans 5 di-D-fructose-2,6':2',6 dianhydride + ?
-
 ?
levan levan from Zymomonas mobilis, conversion yield is 35% Pseudarthrobacter oxydans J17-21 5 di-D-fructose-2,6':2',6 dianhydride + ?
-
 ?
additional information no activity with inulin, levanbiose, sucrose, dextran or starch Pseudarthrobacter oxydans ?
-
 ?
additional information no activity with inulin, levanbiose, sucrose, dextran or starch Pseudarthrobacter oxydans J17-21 ?
-
 ?

Synonyms

Synonyms Comment Organism
LFTase
-
 Pseudarthrobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 30 min, enzyme retains 87% of initial activity Pseudarthrobacter oxydans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 30°C: about 35% of maximal activity, 50°C: about 80% of maximal activity, 60°C: about 15% of maximal activity Pseudarthrobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Pseudarthrobacter oxydans

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 pH 5.0: about 35% of maximal activity, pH 7.5: about 40% of maximal activity Pseudarthrobacter oxydans