General Stability | Organism |
---|---|
1 mM NaCl or 10 mM calcium acetate stabilize against thermal inactivation at 50°C | Gracilariopsis chorda |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride | - |
Gracilariopsis chorda | |
2,4,6-trinitrobenzene-sulfonyl acid | - |
Gracilariopsis chorda | |
Cu2+ | 10 mM, complete inactivation | Gracilariopsis chorda | |
Hg2+ | 1 mM, complete inactivation | Gracilariopsis chorda | |
additional information | no effect with 1 mM Mg2+, Zn2+, Fe2+ or Co2+ | Gracilariopsis chorda | |
N-bromosuccinimide | - |
Gracilariopsis chorda |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
amylopectin | pH 5.0, 35°C, barley amylopectin or potato amylopectin | Gracilariopsis chorda | |
0.03 | - |
glycogen | pH 5.0, 35°C, rabbit liver glycogen | Gracilariopsis chorda | |
0.1 | - |
maltotetraose | pH 5.0, 35°C | Gracilariopsis chorda | |
0.1 | - |
maltopentaose | pH 5.0, 35°C | Gracilariopsis chorda | |
0.1 | - |
maltohexaose | pH 5.0, 35°C | Gracilariopsis chorda | |
0.1 | - |
maltoheptaose | pH 5.0, 35°C | Gracilariopsis chorda | |
0.4 | - |
maltotriose | pH 5.0, 35°C | Gracilariopsis chorda | |
2.3 | - |
maltose | pH 5.0, 35°C | Gracilariopsis chorda |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1.3fold activation | Gracilariopsis chorda |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
x * 100000, SDS-PAGE | Gracilariopsis chorda |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gracilariopsis chorda | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gracilariopsis chorda |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin | barley amylopectin and potato amylopectin | Gracilariopsis chorda | ? | - |
? | |
Glycogen | rabbit liver glycogen | Gracilariopsis chorda | ? | - |
? | |
Maltoheptaose | - |
Gracilariopsis chorda | ? | - |
? | |
Maltohexaose | - |
Gracilariopsis chorda | ? | - |
? | |
maltopentaose | - |
Gracilariopsis chorda | ? | - |
? | |
maltose | - |
Gracilariopsis chorda | D-glucose + 1,5-anhydro-D-fructose | - |
? | |
Maltotetraose | - |
Gracilariopsis chorda | ? | - |
? | |
maltotriose | - |
Gracilariopsis chorda | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, SDS-PAGE | Gracilariopsis chorda |
Synonyms | Comment | Organism |
---|---|---|
GLase | - |
Gracilariopsis chorda |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
optimal activity without addition of any salt | Gracilariopsis chorda |
50 | - |
optimal activity in presence of 1 mM calcium chloride | Gracilariopsis chorda |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 55 | 35°C: about 50% of maximal activity, 55°C: about 80% of maximal activity, optimal activity in presence of 1 mM calcium chloride | Gracilariopsis chorda |
35 | 50 | 35°C: about 70% of maximal activity, 50°C: about 70% of maximal activity, activity without addition of any salt | Gracilariopsis chorda |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 5.0, 20 min, 77% loss of activity without addition of any salt, 49% loss of activity in presence of 1 mM NaCl, 41% loss of activity in presence of 10 mM calcium acetate | Gracilariopsis chorda |