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Literature summary for 4.2.2.13 extracted from

  • Yu, S.; Christensen, T.M.I.E.; Kragh, K.M.; Bojsen, K.; Marcussen, J.
    Efficient purification, characterization and partial amino acid sequencing of two alpha-1,4-glucan lyases from fungi (1997), Biochim. Biophys. Acta, 1339, 311-320.
    View publication on PubMed

General Stability

General Stability Organism
glycogen stabilizes at higher temperatures Morchella costata
glycogen stabilizes at higher temperatures Morchella vulgaris
less than 10% loss of activity by freezing at -20°C overnight and thawing, at a protein concentration of 0.07 mg/ml in 15 mM NaCl Morchella costata
less than 10% loss of activity by freezing at -20°C overnight and thawing, at a protein concentration of 0.07 mg/ml in 15 mM NaCl Morchella vulgaris

Inhibitors

Inhibitors Comment Organism Structure
acarbose
-
Morchella costata
deoxynojirimycin with amylopectin or glycogen as substrate Morchella costata
glucose with amylopectin as substrate. No effect with glycogen as substrate Morchella costata
maltitol with amylopectin or glycogen as substrate Morchella costata
methyl alpha-glucoside with glycogen as substrate Morchella costata
PCMB with amylopectin or glycogen as substrate Morchella costata

Organism

Organism UniProt Comment Textmining
Morchella costata
-
-
-
Morchella vulgaris
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein no detectable amount of carbohydrate Morchella costata
no glycoprotein no detectable amount of carbohydrate Morchella vulgaris

Purification (Commentary)

Purification (Comment) Organism
-
Morchella costata
-
Morchella vulgaris

Storage Stability

Storage Stability Organism
4°C, bis-Tris propane-HCl buffer, pH 7.0, stable for several days Morchella costata
4°C, bis-Tris propane-HCl buffer, pH 7.0, stable for several days Morchella vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Amylopectin
-
Morchella costata Glucose + 1,5-anhydro-D-fructose
-
?
Amylopectin
-
Morchella vulgaris Glucose + 1,5-anhydro-D-fructose
-
?
Glycogen best substrate Morchella costata ?
-
?
Glycogen best substrate Morchella vulgaris ?
-
?
Maltosaccharides
-
Morchella costata Glucose + 1,5-anhydro-D-fructose
-
?
Maltosaccharides
-
Morchella vulgaris Glucose + 1,5-anhydro-D-fructose
-
?
Maltose
-
Morchella costata Glucose + 1,5-anhydro-D-fructose
-
?
Maltose
-
Morchella vulgaris Glucose + 1,5-anhydro-D-fructose
-
?
additional information no activity towards glucans with alpha-1,1-linkages, alpha-1,3-linkages or alpha-1,6-linkages Morchella costata ?
-
?
additional information no activity towards glucans with alpha-1,1-linkages, alpha-1,3-linkages or alpha-1,6-linkages Morchella vulgaris ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
amylopectin as substrate Morchella costata
40
-
glycogen as substrate Morchella costata
43
-
amylopectin as substrate Morchella vulgaris
48
-
glycogen as substrate Morchella vulgaris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60 25°C: about 40% of maximal activity, 60°C: about 40% of maximal activity, glycogen as substrate Morchella vulgaris
25 55 25°C: about 60% of maximal activity, 55°C: about 55% of maximal activity, glycogen as substrate Morchella costata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Morchella costata
6.5
-
-
Morchella vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8 pH 4.5: about 40% of maximal activity, pH 8.0: about 55% of maximal activity Morchella costata
5 8 pH 5.0: about 60% of maximal activity, pH 8.0: about 50% of maximal activity Morchella vulgaris