General Stability | Organism |
---|---|
glycogen stabilizes at higher temperatures | Morchella costata |
glycogen stabilizes at higher temperatures | Morchella vulgaris |
less than 10% loss of activity by freezing at -20°C overnight and thawing, at a protein concentration of 0.07 mg/ml in 15 mM NaCl | Morchella costata |
less than 10% loss of activity by freezing at -20°C overnight and thawing, at a protein concentration of 0.07 mg/ml in 15 mM NaCl | Morchella vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acarbose | - |
Morchella costata | |
deoxynojirimycin | with amylopectin or glycogen as substrate | Morchella costata | |
glucose | with amylopectin as substrate. No effect with glycogen as substrate | Morchella costata | |
maltitol | with amylopectin or glycogen as substrate | Morchella costata | |
methyl alpha-glucoside | with glycogen as substrate | Morchella costata | |
PCMB | with amylopectin or glycogen as substrate | Morchella costata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Morchella costata | - |
- |
- |
Morchella vulgaris | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | no detectable amount of carbohydrate | Morchella costata |
no glycoprotein | no detectable amount of carbohydrate | Morchella vulgaris |
Purification (Comment) | Organism |
---|---|
- |
Morchella costata |
- |
Morchella vulgaris |
Storage Stability | Organism |
---|---|
4°C, bis-Tris propane-HCl buffer, pH 7.0, stable for several days | Morchella costata |
4°C, bis-Tris propane-HCl buffer, pH 7.0, stable for several days | Morchella vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Amylopectin | - |
Morchella costata | Glucose + 1,5-anhydro-D-fructose | - |
? | |
Amylopectin | - |
Morchella vulgaris | Glucose + 1,5-anhydro-D-fructose | - |
? | |
Glycogen | best substrate | Morchella costata | ? | - |
? | |
Glycogen | best substrate | Morchella vulgaris | ? | - |
? | |
Maltosaccharides | - |
Morchella costata | Glucose + 1,5-anhydro-D-fructose | - |
? | |
Maltosaccharides | - |
Morchella vulgaris | Glucose + 1,5-anhydro-D-fructose | - |
? | |
Maltose | - |
Morchella costata | Glucose + 1,5-anhydro-D-fructose | - |
? | |
Maltose | - |
Morchella vulgaris | Glucose + 1,5-anhydro-D-fructose | - |
? | |
additional information | no activity towards glucans with alpha-1,1-linkages, alpha-1,3-linkages or alpha-1,6-linkages | Morchella costata | ? | - |
? | |
additional information | no activity towards glucans with alpha-1,1-linkages, alpha-1,3-linkages or alpha-1,6-linkages | Morchella vulgaris | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
amylopectin as substrate | Morchella costata |
40 | - |
glycogen as substrate | Morchella costata |
43 | - |
amylopectin as substrate | Morchella vulgaris |
48 | - |
glycogen as substrate | Morchella vulgaris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | 25°C: about 40% of maximal activity, 60°C: about 40% of maximal activity, glycogen as substrate | Morchella vulgaris |
25 | 55 | 25°C: about 60% of maximal activity, 55°C: about 55% of maximal activity, glycogen as substrate | Morchella costata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Morchella costata |
6.5 | - |
- |
Morchella vulgaris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | pH 4.5: about 40% of maximal activity, pH 8.0: about 55% of maximal activity | Morchella costata |
5 | 8 | pH 5.0: about 60% of maximal activity, pH 8.0: about 50% of maximal activity | Morchella vulgaris |