Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1 mM, slight stimulation | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, 30% loss of activity | Bacillus sp. (in: Bacteria) | |
HgCl2 | 1 mM, 95% loss of activity | Bacillus sp. (in: Bacteria) | |
iodoacetamide | 1 mM, 70% loss of activity | Bacillus sp. (in: Bacteria) | |
NaCl | 150 mM, 70% loss of activity | Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | excreted to medium | Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, slight stimulation | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
enzyme is induced by xanthan | - |
Bacillus sp. (in: Bacteria) GL1 | - |
enzyme is induced by xanthan | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.28 | - |
pH 7.0 | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvylated xanthan | best substrate | Bacillus sp. (in: Bacteria) | pyruvylate mannose + oligosaccharides | - |
? | |
pyruvylated xanthan | best substrate | Bacillus sp. (in: Bacteria) GL1 | pyruvylate mannose + oligosaccharides | - |
? | |
xanthan | - |
Bacillus sp. (in: Bacteria) | oligosaccharides | - |
? | |
xanthan | - |
Bacillus sp. (in: Bacteria) GL1 | oligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 75000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
pH 7.0, 10 min, loss of 60% of activity | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | at 4°C | Bacillus sp. (in: Bacteria) |