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Literature summary for 4.2.2.11 extracted from
- Purification and characterisation of a bifunctional alginate lyase from novel Isoptericola halotolerans CGMCC 5336 (2013), Carbohydr. Polym., 98, 1476-1482.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, phylogenetic analysis | Isoptericola halotolerans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
alginate | Km value 0.26 mg/ml, pH 7.0, 30°C | Isoptericola halotolerans |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Isoptericola halotolerans | |
| Ca2+ | 158% of initial activity | Isoptericola halotolerans | |
| Mg2+ | activates | Isoptericola halotolerans | |
| Mg2+ | 173% of initial activity | Isoptericola halotolerans | |
| Mn2+ | activates | Isoptericola halotolerans | |
| Na+ | activates | Isoptericola halotolerans | |
| Na+ | optimum concentration 4 M, about 25% activation | Isoptericola halotolerans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
- |
Isoptericola halotolerans |
| 28000 | - |
x * 28000, SDS-PAGE | Isoptericola halotolerans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate | Isoptericola halotolerans | the bifunctional alginate lyase shows substrate specificity for poly(alpha-L-guluronate) and poly(beta-D-mannuronate) units in alginate molecules, cf. EC 4.2.2.3 | ? | - |
? | |
| alginate | Isoptericola halotolerans CGMCC5336 | the bifunctional alginate lyase shows substrate specificity for poly(alpha-L-guluronate) and poly(beta-D-mannuronate) units in alginate molecules, cf. EC 4.2.2.3 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Isoptericola halotolerans | - |
- |
- |
| Isoptericola halotolerans | AB489222 | isolated from rotten seaweed | - |
| Isoptericola halotolerans CGMCC 5336 | - |
- |
- |
| Isoptericola halotolerans CGMCC5336 | AB489222 | isolated from rotten seaweed | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 1.75fold by ammonium sulfate fractionation and anion exchange chromatography | Isoptericola halotolerans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8409 | - |
pH 7.0, 30°C | Isoptericola halotolerans |
| 8409 | - |
purified native enzyme, pH 7.0, 50°C | Isoptericola halotolerans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate | the bifunctional alginate lyase shows substrate specificity for poly(alpha-L-guluronate) and poly(beta-D-mannuronate) units in alginate molecules, cf. EC 4.2.2.3 | Isoptericola halotolerans | ? | - |
? | |
| alginate | enzyme shows specificity for polyguluronate and polymannuronate units in alginate molecules | Isoptericola halotolerans | ? | - |
? | |
| alginate | the bifunctional alginate lyase shows substrate specificity for poly(alpha-L-guluronate) and poly(beta-D-mannuronate) units in alginate molecules, cf. EC 4.2.2.3 | Isoptericola halotolerans CGMCC5336 | ? | - |
? | |
| alginate | enzyme shows specificity for polyguluronate and polymannuronate units in alginate molecules | Isoptericola halotolerans CGMCC 5336 | ? | - |
? | |
| additional information | no substrats: pectin, xanthan, guar gum, arabic gum, sesbania gum, guar gum and carrageenan | Isoptericola halotolerans | ? | - |
? | |
| additional information | no substrats: pectin, xanthan, guar gum, arabic gum, sesbania gum, guar gum and carrageenan | Isoptericola halotolerans CGMCC 5336 | ? | - |
? | |
| poly(alpha-L-guluronate) | - |
Isoptericola halotolerans | ? | - |
? | |
| poly(alpha-L-guluronate) | - |
Isoptericola halotolerans CGMCC5336 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000, SDS-PAGE | Isoptericola halotolerans |
| monomer | 1 * 28000 | Isoptericola halotolerans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Isoptericola halotolerans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 60 | activity range, profile overview | Isoptericola halotolerans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable below | Isoptericola halotolerans |
| 40 | - |
purified enzyme, loss of 40% activity after 180 min | Isoptericola halotolerans |
| 50 | - |
purified enzyme, inactivation after 120 min | Isoptericola halotolerans |
| 60 | - |
purified enzyme, inactivation after 90 min | Isoptericola halotolerans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Isoptericola halotolerans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | activity range, profile overview | Isoptericola halotolerans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | 12 h, more than 60% residual activity | Isoptericola halotolerans |
| 5.5 | 8.5 | purified enzyme, 12 h, over 60% activity within this pH range remains pH 5.5 to 8.5, mostly stable at pH 7.5, half inactivation at pH 9.5 | Isoptericola halotolerans |
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