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Literature summary for 4.2.2.11 extracted from
- Molecular identification of a polyM-specific alginate lyase from Pseudomonas sp. strain KS-408 for degradation of glycosidic linkages between two mannuronates or mannuronate and guluronate in alginate (2011), Can. J. Microbiol., 57, 1032-1041.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pseudomonas sp. |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| homology modeling of structure. Residues Asn198, His199, Arg246, and Tyr253 are conserved for the catalytic active site | Pseudomonas sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42400 | - |
x * 45500, recombinant enzyme with signal peptide, x * 42400, recombinant enzyme without signal peptide, calulated | Pseudomonas sp. |
| 45500 | - |
x * 45500, recombinant enzyme with signal peptide, x * 42400, recombinant enzyme without signal peptide, calulated | Pseudomonas sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | G9J5R3 | - |
- |
| Pseudomonas sp. KS-408 | G9J5R3 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | recombinant enzyme with signal peptide and without signal peptide with the His-tag consist of 393 and 372 amino acids, respectively | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate | - |
Pseudomonas sp. | ? | enzyme cleaves the glycosidic linkages between two mannuronates (mannuronate-beta(1-4)-mannuronate) or mannuronate and guluronate (mannuronate-beta(1-4)-guluronate) | ? |  |
| alginate | - |
Pseudomonas sp. KS-408 | ? | enzyme cleaves the glycosidic linkages between two mannuronates (mannuronate-beta(1-4)-mannuronate) or mannuronate and guluronate (mannuronate-beta(1-4)-guluronate) | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45500, recombinant enzyme with signal peptide, x * 42400, recombinant enzyme without signal peptide, calulated | Pseudomonas sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Aly1 | - |
Pseudomonas sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable for at least 30 min | Pseudomonas sp. |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas sp. | recombinant enzyme without signal peptide, calculated | - |
8.5 |
| Pseudomonas sp. | recombinant enzyme with signal peptide, calculated | - |
8.6 |
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