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Literature summary for 4.2.2.11 extracted from

  • Inoue, A.; Mashino, C.; Kodama, T.; Ojima, T.
    Protoplast preparation from Laminaria japonica with recombinant alginate lyase and cellulase (2011), Mar. Biotechnol., 13, 256-263.
    View publication on PubMed
Search for more literature for 4.2.2.11

Application

Application Comment Organism
additional information use of recombinant abalone alginate lyase and beta-1,4-endoglucanase for protoplast isolation from Laminaria japonica. In Laminaria japonica blades pretreated with proteinase K and incubated in artificial seawater containing alginate lyase and beta-1,4-endoglucanase, the protoplast number is increased up to 5000000 protoplasts/g fresh weight. These cells are mostly derived from the epidermal layer rather than the cortical layer. Results suggest that at least three enzymes, alginate lyase, cellulase, and protease, are essential for effective protoplast isolation from Laminaria japonica Haliotis discus hannai

Cloned(Commentary)

Cloned (Comment) Organism
baculoviral expression system Haliotis discus hannai

Organism

Organism UniProt Comment Textmining
Haliotis discus hannai
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2490
-
 30°C, pH 8.0 Haliotis discus hannai

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-L-guluronosyl linkage in alginate
-
 Haliotis discus hannai ?
-
 ?

Synonyms

Synonyms Comment Organism
oligoalginate lyase
-
 Haliotis discus hannai

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Haliotis discus hannai

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, 50% residual activity Haliotis discus hannai

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
-
 Haliotis discus hannai