Literature summary for 4.2.2.11 extracted from

Matsushima, R.; Danno, H.; Uchida, M.; Ishihara, K.; Suzuki, T.; Kaneniwa, M.; Ohtsubo, Y.; Nagata, Y.; Tsuda, M.
Analysis of extracellular alginate lyase and its gene from a marine bacterial strain, Pseudoalteromonas atlantica AR06 (2010), Appl. Microbiol. Biotechnol., 86, 567-576.

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Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	1 mM, 3fold decrease in activity	Pseudoalteromonas atlantica

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Pseudoalteromonas atlantica	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	50 mM, 10% increase in activity	Pseudoalteromonas atlantica
Mg2+	50 mM, 2fold increase in activity	Pseudoalteromonas atlantica
Na+	50 mM, 10% increase in activity	Pseudoalteromonas atlantica

Organism

Organism	UniProt	Comment	Textmining
Pseudoalteromonas atlantica	D1MX66	-	-
Pseudoalteromonas atlantica AR06	D1MX66	-	-

Subunits

Subunits	Comment	Organism
More	transcription of the alyA gene leads to a 43-kDa protein, which is the derivative of the nascent alyA gene product lacking the N-terminal 22 amino-acid signal peptide, and to 33- and 30.5-kDa proteins, which are the C-terminal portions of the nascent alyA gene products that start from the amino-acid residue positions 148 and 162, respectively	Pseudoalteromonas atlantica

Synonyms

Synonyms	Comment	Organism
AlyA	-	Pseudoalteromonas atlantica

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Pseudoalteromonas atlantica

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	-	50% of maximum activity	Pseudoalteromonas atlantica
50	-	30% of maximum activity	Pseudoalteromonas atlantica

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	-	Pseudoalteromonas atlantica

General Information

General Information	Comment	Organism
physiological function	knockout mutant shows growth rates similar to wild-type in MB medium, but grows very slowly in the ASW liquid medium supplemented with 0.3% alginate	Pseudoalteromonas atlantica

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Release 2023.1 (January 2023)