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Literature summary for 4.2.2.11 extracted from
- Purification and characterization of endo poly (alpha-L-guluronate) lyase in the enzyme system from Flavobacterium multivorum (1997), Food Sci. Technol. Int., 3, 22-26.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Sphingobacterium multivorum |  |
| N-bromosuccinimide | - |
Sphingobacterium multivorum | |
| p-chloromercuribenzoate | - |
Sphingobacterium multivorum | |
| trinitrobenzene sulfonic acid | - |
Sphingobacterium multivorum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
gel filtration | Sphingobacterium multivorum |
| 43000 | - |
1 * 43000, SDS-PAGE | Sphingobacterium multivorum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly-alpha-L-guluronic acid | Sphingobacterium multivorum | - |
unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate | - |
? | |
| poly-alpha-L-guluronic acid | Sphingobacterium multivorum K-11 | - |
unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sphingobacterium multivorum | - |
K-11 | - |
| Sphingobacterium multivorum K-11 | - |
K-11 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cation exchange chromatography, chromatofocusing and gel filtration | Sphingobacterium multivorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-L-guluronosyl linkage in alginate | endolyase | Sphingobacterium multivorum | ? | - |
? | |
| alpha-L-guluronosyl linkage in alginate | endolyase | Sphingobacterium multivorum K-11 | ? | - |
? | |
| poly-alpha-L-guluronic acid | - |
Sphingobacterium multivorum | ? | - |
? | |
| poly-alpha-L-guluronic acid | - |
Sphingobacterium multivorum K-11 | ? | - |
? | |
| poly-alpha-L-guluronic acid | - |
Sphingobacterium multivorum | unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate | - |
? | |
| poly-alpha-L-guluronic acid | - |
Sphingobacterium multivorum K-11 | unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Sphingobacterium multivorum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Sphingobacterium multivorum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable at temperatures up to 30 °C | Sphingobacterium multivorum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Sphingobacterium multivorum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Sphingobacterium multivorum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Sphingobacterium multivorum | - |
- |
8.7 |
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